共轭亚油酸对小鼠肥胖的抑制作用

doi:10.7506/spkx1002-6630-201603038

摘要/Abstract

摘要：

采用小鼠营养性肥胖模型法，以昆明小鼠为实验动物，设置基础饲料对照组、肥胖模型对照组、共轭亚油酸（conjugated linoleic acid，CLA）高、中、低剂量组，分别连续灌胃6 周，考察小鼠体质量、体内脂肪质量、血脂水平、肝脏脂肪酸合成酶（fatty acid synthase，FAS）含量及脏器的变化，研究CLA对小鼠肥胖的抑制作用。结果表明：CLA各剂量组小鼠的Lee’s指数、脂肪系数和血清甘油三酯（triglyceride，TG）、总胆固醇（totalcholesterol，TC）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）水平均显著或极显著低于肥胖模型对照组（P＜0.05或P＜0.01），高密度脂蛋白胆固醇（high density lipoprotein cholesterol，HDL-C）水平均极显著高于肥胖模型对照组（P＜0.01），各剂量的CLA对小鼠除肝脏以外的其他脏器无显著影响（P＞0.05），高剂量（0.15 mL/10 g）CLA可使喂食营养饲料小鼠的各项肥胖指标均处于喂食基础饲料小鼠的水平，表明CLA能有效抑制小鼠肥胖，同时对小鼠生长无毒副作用；CLA可极显著降低小鼠肝脏FAS含量（P＜0.01），降低脂肪酸的合成，从而抑制小鼠肥胖。

关键词: 共轭亚油酸, 抑制肥胖, 脂肪酸合成酶

Abstract:

Conjugated linoleic acid (CLA), a mixture of positional and geometric isomers of linoleic acid with conjugated
double bonds, has attracted considerable attention since cis-9, trans-11-CLA (c9, t11-CLA) and trans-10, cis-12-CLA
(t10, c12-CLA) have a great application potential in medicines, foods and health products based on anticancer, immune
regulatory, anti-atherosclerosis, anti-obesity and antioxidant activities. In this study, Kunming mice were used as
experimental subjects to evaluate the inhibitory effect of CLA (80% purity, containing 36.40% c9, t11-CLA, 36.26%
t10, c12-CLA and 7.14% other CLA isomers) on the obesity of mice. The tested mice were divided into obesity model
control group, normal control group and CLA treatment group, and the mice in the CLA treatment group were administered
with CLA by gavage for six weeks, and then obesity indexes, such as body weight, Lee’s index, total weight of fat, fatty
coefficient, and total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density
lipoprotein cholesterol (HDL-C) in serum, fatty acid synthase (FAS) in liver and viscera of the mice, were measured. The
results showed that the obesity indexes, including Lee’s index, fatty coefficient, TC, TG and LDL-C, in the CLA treatment
groups at various dosages were significantly lower than the obesity model control group (P < 0.05 or P < 0.01), and HDL-C
was significantly higher than the obesity model control group (P < 0.01). Lee’s index, fatty coefficient, TC, TG, and LDL-C
decreased with increasing CLA dose, while HDL-C increased. High-dose CLA (0.15 mL/10 g) could control the obesity
indexes of obesity model control group to the level of the normal control group, and CLA treatment at each dose had no
significant effect on the viscera of the mice; therefore, CLA could inhibit obesity and had no toxic side effect on the growth
of mice. The Lee’s index, fatty coefficient, TC, TG, and LDL-C had high positive correlation with FAS, while HDL-C
had a highly negative correlation. An obvious correlation between FAS and obesity index was observed and it was further
confirmed that FAS is the potential target of obesity. CLA treatment can significantly reduce FAS (P < 0.01), and this could
be the mechanism of its antiobesity effect.

Key words: conjugated linoleic acid (CLA), obesity inhibition, fatty acid synthase (FAS)

中图分类号:

TS201.2

王武，李琪玲，潘见. 共轭亚油酸对小鼠肥胖的抑制作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201603038.

WANG Wu, LI Qiling, PAN Jian. Inhibitory Effect of Conjugated Linoleic Acid on Obesity of Mice[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201603038.

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共轭亚油酸对小鼠肥胖的抑制作用

Inhibitory Effect of Conjugated Linoleic Acid on Obesity of Mice

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