食品科学

• 工艺技术 • 上一篇    下一篇

响应面试验优化中性蛋白酶辅助提取青稞淀粉工艺

赵彦巧,朱志勇,李建颖,张丽君   

  1. 天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津 300134
  • 出版日期:2016-02-25 发布日期:2016-02-23
  • 基金资助:

    天津市高校科技发展基金项目(140023);天津市高等学校“十二五”综合投资人才培养项目(507-125RCPY0315);
    天津市教育科学“十二五”规划重点研究课题(HE3024);天津市科学技术普及项目(13KPXM01SY008);
    天津市科技型中小企业技术创新资金项目(13ZXCXSY08400)

Optimization of Neutral Protease-Assisted Extraction of Highland Barley Starch

ZHAO Yanqiao, ZHU Zhiyong, LI Jianying, ZHANG Lijun   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
    Tianjin University of Commerce, Tianjin 300134, China
  • Online:2016-02-25 Published:2016-02-23

摘要:

采用中性蛋白酶辅助提取青稞淀粉,研究料液比、加酶量、酶解时间、酶解温度和pH值对青稞淀粉中蛋白残留量的影响,选择加酶量、酶解时间、酶解温度为影响因素进行响应面优化试验。以淀粉蛋白残留量和淀粉提取率为评价指标,确定最佳提取工艺条件。结果表明,加酶量、酶解温度、酶解时间、加酶量与酶解温度的交互作用及加酶量与酶解时间的交互作用对淀粉蛋白残留量有极显著影响,而对淀粉提取率无显著影响。实验范围内得到的最佳提取工艺条件为加酶量140.79 U/g、酶解温度45.01 ℃、酶解时间2.57 h,在此条件下青稞淀粉的提取率为60.36%,淀粉蛋白残留量为1.31%。

关键词: 青稞淀粉, 中性蛋白酶, 蛋白残留, 响应面

Abstract:

The neutral protease-assisted extraction of highland barley starch was optimized using response surface
methodology. The effects of several operating parameters, namely solid to liquid ratio, enzyme dosage, hydrolysis time,
temperature and pH, on the residual protein content of highland barley starch were studied. Three main factors including
enzyme dosage, hydrolysis time and temperature were chosen to design response surface experiments using residual protein
content and starch yield as the responses. The results showed that the three selected factors as well as the interaction between
enzyme dosage and either hydrolysis time or temperature all had significant effects on residual protein content, but had no
significant effects on starch yield. The optimal extraction conditions in the selected experimental ranges were determined as
follows: enzyme dosage, 140.79 U/g; temperature, 45.01 ℃; and hydrolysis time, 2.57 h, leading to a starch yield of 60.36%
and a residual protein content of 1.31%.

Key words: highland barley starch, neutral protease, residual protein, response surface methodology

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