关键词: 利巴韦林, 代谢, 蛋鸡, 超高效液相色谱-串联质谱法

Abstract:

This study aimed to examine the residues of the banned antiviral drug ribavirin in poultry. Eleven Jinghong laying
hens were fed with 30 mg/kg BW ribavirin via single oral administration and their blood samples were collected from a vein
under the wing at different time points. The ribavirin in the plasma samples was measured by using ultra high performance
liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring mode (MRM)
and quantified using isotope-labeled internal standard. The calibration curve for ribavirin exhibited good linearity over the
concentration range from 5 to 5 000 ng/mL, and the limit of detection (LOD) was 1 ng/mL. Average recoveries for ribavirin
spiked at 3 levels ranged from 86.4% to 97.1%, and the inter-batch and intra-batch relative standard deviations (RSDs) both
met the quantitative requirement. Ribavirin concentration in laying hens plasma increased rapidly after oral administration
but then decreased gradually with metabolic elimination. The results suggested that the pharmacokinetic characteristics of
ribavirin in hens include quick absorption, broad distribution, rapid elimination, and lower residue in tissue.

Key words: ribavirin, metabolism, laying hens, ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)