关键词: 葡萄干, 总亚硫酸盐, 荧光衍生化法

Abstract:

A rapid and accurate method for the determination of total sulfite in Xinjiang specialty raisin was developed using
fluorescence derivatization. N-(9-Acridinyl) maleimide (NAM) reacted with the sulfite in raisin to form strong fluorescent
derivatives, which were then detected by fluorometry. The linear range of the method was between 1.0 and 43.2 mg/L, with a
correlation coefficient (R2) of 0.999 3. At three spiked levels, the average recoveries of sulfite in raisin ranged from 92.2% to
100.2% with relative standard deviations lower than 3.80% (n = 3). Furthermore, the results obtained by this method for the
total sulfite content of raisin were consistent with those obtained by the pararosaniline method. These results indicate that the
fluorescence derivatization method can accurately and rapidly determine total sulfite in Xinjiang specialty raisin.

Key words: raisin, total sulfite, fluorescence derivatization