重庆地区猪肉样品中猪链球菌污染、血清型、耐药性及致病性

doi:10.7506/spkx1002-6630-201604042

食品科学

重庆地区猪肉样品中猪链球菌污染、血清型、耐药性及致病性

彭少静，邓华英，张媛媛，胡书月，丁玲玲，李继祥

西南大学荣昌校区动物医学系，重庆 402460

出版日期:2016-02-25 发布日期:2016-02-23
通讯作者: 李继祥

Contamination, Serotype, Drug Resistance and Pathogenicity of Streptococcus suis from Pork Samples in Chongqing

PENG Shaojing, DENG Huaying, ZHANG Yuanyuan, HU Shuyue, DING Lingling, LI Jixiang

Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, China

Online:2016-02-25 Published:2016-02-23
Contact: LI Jixiang

摘要/Abstract

摘要：

采集于重庆地区屠宰场的猪颌下淋巴结398 份和农贸市场猪肉样品140 份，经选择增菌培养后分离出猪链球菌132 株，平均检出率为24.5%（132/538），其中屠宰场和农贸市场样品的检出率分别为26.7%（106/398）和18.6%（26/140）。经血清型特异性聚合酶链式反应检测，样品中猪链球菌血清1型、2型、7型和9型菌株的检出率分别3.16%（17/538）、3.16%（17/538）、6.88%（37/538）和1.67%（9/538）；药敏纸片法检测18 株细菌的耐药性，发现对青霉素类和头孢菌素类药物100%敏感，对氨基糖苷类、四环素类和磺胺类药物耐药性严重；小白鼠用于部分菌株的感染实验，结果显示分离菌株均有致病性。研究结果表明，屠宰生猪及农贸市场猪肉存在多种血清型的致病性猪链球菌污染。

关键词: 猪链球菌, 猪肉, 血清型, 耐药性, 致病性

Abstract:

A total of 132 strains of Streptococcus suis (S. suis) were isolated from 398 submaxillary lymphonodi samples
collected from pig slaughterhouses and 140 pork samples collected from farmers’ markets in Chongqing. The average
contamination rate of S. suis in the total samples was 24.5% (132/538). In addition, the S. suis contamination rates of
submaxillary lymphonodi and pork were 26.7% (106/398) and 18.6% (26/140), respectively. The serotype-specific PCR
assays were used to identify the serotypes and the detection rates of serotype 1, 2, 7, and 9 were 3.16% (17/538), 3.16%
(17/538), 6.88% (37/538) and 1.67% (9/538) in samples, respectively. The drug resistance of 18 isolates was evaluated
by K-B method. The results exhibited that these isolates were strongly tolerant to aminoglycosides, tetracyclines and
sulfonamides, but sensitive to penicillins and cephalosporins. Infection experiments in mice were conducted to determine the
virulence of 15 of the isolates. It was shown that these isolates were pathogenic differently to mice. The results of this study
indicated that much attention should be paid to multi-serotype S. suis contamination of pig carcass and pork in quarantine.

Key words: Streptococcus suis, pork, serotype, drug resistance, pathogenicity

中图分类号:

TS201.6

彭少静，邓华英，张媛媛，胡书月，丁玲玲，李继祥. 重庆地区猪肉样品中猪链球菌污染、血清型、耐药性及致病性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201604042.

PENG Shaojing, DENG Huaying, ZHANG Yuanyuan, HU Shuyue, DING Lingling, LI Jixiang. Contamination, Serotype, Drug Resistance and Pathogenicity of Streptococcus suis from Pork Samples in Chongqing[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201604042.

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重庆地区猪肉样品中猪链球菌污染、血清型、耐药性及致病性

Contamination, Serotype, Drug Resistance and Pathogenicity of Streptococcus suis from Pork Samples in Chongqing

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