食品科学

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6-苄氨基嘌呤对采后空心菜品质及其活性氧代谢的影响

芦 航1,2,高建晓1,2,胡花丽1,李鹏霞1,*   

  1. 1.江苏省农业科学院农产品加工研究所,江苏 南京 210014;2.南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2016-02-25 发布日期:2016-02-23
  • 通讯作者: 李鹏霞
  • 基金资助:

    江苏省自主创新项目(CX(15)1048)

Effect of 6-Benzylaminopurine Treatment on Postharvest Quality and Reactive Oxygen Species Metabolism of Ipomoea aqua

LU Hang1,2, GAO Jianxiao1,2, HU Huali1, LI Pengxia1,*   

  1. 1. Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China
  • Online:2016-02-25 Published:2016-02-23
  • Contact: LI Pengxia

摘要:

以大叶空心菜为材料,在(10±1)℃、相对湿度为90%~95%条件下进行货架模拟,以清水处理为对照,研究了30 mg/L 6-苄氨基嘌呤(6-benzylaminopurine,6-BA)处理对采后空心菜品质及活性氧代谢的影响。结果表明:30 mg/L 6-BA处理维持了采后空心菜较高的叶绿素含量,提高了超氧化物歧化酶的活性,降低了贮藏4 d和8 d时抗坏血酸过氧化物酶的活性,但其对空心菜过氧化物酶的活性无显著影响;同时,6-BA处理显著推迟了采后空心菜内源性抗氧化物质含量的下降,如还原型抗坏血酸和还原型谷胱甘肽含量的下降;此外,6-BA处理还减缓了空心菜组织中O2-·生成速率及H2O2、丙二醛含量的增加。这些结果表明30 mg/L 6-BA处理可延缓采后空心菜的衰老,从而维持其品质。

关键词: 空心菜, 6-苄氨基嘌呤, 品质, 活性氧代谢

Abstract:

Large-leafed Ipomoea aquatica was pretreated with 0 (as the control) and 30 mg/L 6-benzylaminopurine (6-BA),
and then stored at (10 ± 1) ℃ with 90%–95% relative humidity. Changes in the reactive oxygen species (ROS) metabolism
and quality of Ipomoea aquatica were assessed. The results showed that treatment with 30 mg/L 6-BA maintained higher
content of chlorophyll, increased the activity of superoxide dismutase, and decreased the activity of ascorbate peroxidase in
Ipomoea aquatica after 4 and 8 days of storage. However, no difference in peroxidase activity was observed between 6-BA
treatment and the control. Meanwhile, 6-BA treatment significantly delayed the decrease in endogenous antioxidants of
posthtarvest Ipomoea aquatica. For example, the decline in the contents of ascorbic acid and glutathione was slowed down.
Moreover, compared to the control sample, the increase in the production of superoxide anion radicals, and the contents of
hydrogen peroxide and malonaldehyde in 6-BA-treated samples were mitigated. These results suggested that 6-BA treatment
could alleviate the senescence of postharvest Ipomoea aquatica, thereby maintaining its quality.

Key words: Ipomoea aquatica, 6-benzylaminopurine, quality, active oxygen metabolism

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