离子液体辅助超声萃取-高效液相色谱同时测定半边莲中6种黄酮类化合物

摘要/Abstract

摘要： 摘要：目的：通过单因素试验和正交试验对离子液体辅助萃取半边莲中黄酮类化合物的条件进行优化，建立高效液相色谱法同时分离测定半边莲中芦丁、槲皮素、柚皮素、橙皮素、山柰酚和芹菜素的方法。方法：采用InertSustain C18色谱柱 (150 mm×4.6 mm, 5 μm)进行分离；流动相为甲醇(B)-乙酸水溶液 (A, pH=3.0)，梯度洗脱；流速1.0 mL/min；紫外检测波长为285 nm；柱温35 ℃。结果：以黄酮类化合物的提取总质量为指标，最佳提取条件为甲醇体积分数为80%、固液比为1:80、萃取时间30 min、离子液体浓度为0.6 mol/L。在优化的色谱条件下，芦丁、槲皮素、柚皮素、橙皮素、山柰酚和芹菜素分离效果良好；定性检测限( S/N = 3 )依次为0.0012、0.0023、0.0026、0.0002、0.0010、0.0006 μg/mL；样品回收率为91.77%~102.53%。结论：该萃取方法操作简单快速，为半边莲中黄酮类化合物的提取分离及检测提供了有效的方法。

关键词: 关键词：高效液相色谱, 半边莲, 黄酮类化合物, 离子液体, 超声萃取

Abstract: Abstract： Objective: Single factor and orthogonal array designs were applied to optimize the conditions for ionic liquid-assisted extraction of flavonoids compounds, the high performance liquid chromatography (HPLC) method was established for simultaneous determination of rutin, quercetin, naringgenin, hesperetin, kaempferol and apigenin in Lobelia chinensis Lour.. Methods: The 6 compounds in Lobelia chinensis Lour. were separated on InertSustain C18 (150 mm×4.6 mm, 5 μm). Mobile phase consisted of methanol and acetic acid (pH=3.0) with gradient elution at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 285 nm. The column temperature was maintained at 35 ℃. Results: the optimum extraction conditions for total flavonoids compounds were determined as follows: methanol percentage, 80%; solid to liquid ratio, 1:80; extraction time, 30 min; ionic liquid concentraction, 0.6 mol//L. Under optimal conditions, rutin, quercetin, naringgenin, hesperetin, kaempferol and apigenin were separationed satisfactorily; The limits of detection ( LOD) were 0.0012、0.0023、0.0026、0.0002、0.0010、0.0006 μg/mL, respectively; The recovery ranged from 91.77%~102.53%. Conclusion: The method is simple, rapid, which provides an efficient means for extraction, separation and determination of flavonoids compounds in Lobelia chinensis Lour..

Key words: Key words：High performance liquid chromatography, Lobelia chinensis Lour., Flavonoids compounds, Ionic liquid, Ultrasonic Extraction

中图分类号:

O652.62

陈军华邓永利周光明高意廖安辉. 离子液体辅助超声萃取-高效液相色谱同时测定半边莲中6种黄酮类化合物[J]. 食品科学, 0, (): 0-0.

Guang-Ming ZHOU Yi GAO. Simultaneous Determination of 6 Flavonoids compounds in Lobelia chinensis Lour. By High Performance Liquid Chromatography with Ionic Liquid Assisted Ultrasonic Extraction[J]. FOOD SCIENCE, 0, (): 0-0.

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