干燥方式对金银花多酚组分及其抗氧化活性的影响

doi:10.7506/spkx1002-6630-201605015

摘要/Abstract

摘要：

研究传统热风干燥、自然阴干以及真空干燥、真空冷冻干燥等不同干燥方式对金银花多酚类物质含量及抗氧化活性的影响。采用Folin-酚比色法及高效液相色谱（high performance liquid chromatography，HPLC）法分别检测4 种干燥方式下的金银花总酚及多酚类组分含量，并比较其清除1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基、羟自由基（•OH）和2,2’-二氮-双（3-乙基苯并噻唑-6-磺酸）铵盐（2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonate acid) diammonium salt，ABTS）自由基的能力。结果表明：4 种干燥方式对金银花总酚含量及多酚类组分均有一定影响，其中真空冷冻干燥处理时金银花总酚及多酚类组分损失最小；不同干燥方式对金银花的抗氧化能力影响显著（P＜0.05），真空冷冻干燥样品清除DPPH自由基、•OH和ABTS＋•的半抑制浓度（IC50）分别为5.43、17.64、44.32 μg/mL；而自然阴干、真空干燥和热风干燥处理的样品清除DPPH自由基、•OH及ABTS＋•的IC50高于真空冷冻干燥金银花相应的IC50。相关性分析显示，金银花酚酸类组分与其抗氧化活性显著相关（P＜0.05）。因此，真空冷冻干燥能较好地保留金银花多酚类物质，且具有较强的抗氧化活性，适宜用于金银花的干燥。

关键词: 金银花, 干燥方式, 多酚, 抗氧化活性

Abstract:

The aim of the present study was to evaluate the effects of four drying methods including hot air drying, shade
drying, vacuum drying and vacuum freeze drying on polyphenol compounds and antioxidant activity of Lonicera japonica
flower. The contents of total phenols and individual phenolic compounds were determined by Folin-Ciocalteu and high
performance liquid chromatography (HPLC), respectively. In addition, the free radical scavenging activities of polyphenols
extracted from Lonicera japonica flower against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and 2,2’-azinobis
(3-ethylbenzothiazoline-6-sulfonate acid) diammonium salt (ABTS) radicals were determined. The results showed that the contents
of total phenols and individual phenolic compounds were affected by four drying methods, especially by vacuum freeze drying.
The antioxidant activities of polyphenols extracted from Lonicera japonica flower were also significantly affected by four drying
methods (P < 0.05). The IC50 of vacuum freeze dried Lonicera japonica for scavenging capacity against DPPH, hydroxyl and
ABTS radicals were 5.43, 17.64 and 44.32 μg/mL, respectively. It was clearly indicated that the IC50 of hot air dried, shade dried
and vacuum dried Lonicera japonica was higher than that of vacuum freeze dried sample. Correlation analysis exhibited a high
relationship between phenolic acid and antioxidant activity (P < 0.05). Based on these results, vacuum freeze drying is the most
suitable method for retaining phenolic compounds and antioxidant activities in Lonicera japonica.

Key words: Lonicera japonica, drying methods, polyphenols, antioxidant activity

中图分类号:

TS201.4

邹容，游玉明，陈泽雄，胡凯，冉烈. 干燥方式对金银花多酚组分及其抗氧化活性的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201605015.

ZOU Rong, YOU Yuming, CHEN Zexiong, HU Kai, RAN Lie. Effects of Drying Methods on Polyphenol Compounds and Antioxidant Activities of Lonicera japonica Flower[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201605015.

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干燥方式对金银花多酚组分及其抗氧化活性的影响

Effects of Drying Methods on Polyphenol Compounds and Antioxidant Activities of Lonicera japonica Flower

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