食品科学

• 基础研究 • 上一篇    下一篇

大孔吸附树脂对草鱼蛋白水解液脱盐的作用

陈丽丽,赵 利*,袁美兰,白春清,冯 飞,王 璐   

  1. 江西科技师范大学生命科学学院,国家大宗淡水鱼加工技术研发分中心,江西省生物加工过程重点实验室,江西 南昌 330013
  • 出版日期:2016-03-15 发布日期:2016-03-17

Desalination of Grass Carp Protein Hydrolysate Using Macroporous Adsorption Resin

CHEN Lili, ZHAO Li*, YUAN Meilan, BAI Chunqing, FENG Fei, WANG Lu   

  1. Jiangxi Province Key Laboratory for Bioprocess Processing, National R&D Branch Center for Freshwater Fish Processing, College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  • Online:2016-03-15 Published:2016-03-17

摘要:

为了脱除草鱼蛋白水解液中的盐,本研究筛选了4 种型号大孔吸附树脂DA201-C、DA201-M、SQT-67和D002,对水解液中草鱼多肽的吸附能力进行测定,并进行静态吸附和解吸实验。结果表明:大孔吸附树脂DA201-C对草鱼多肽的吸附效果较好,其吸附量随草鱼多肽质量浓度的增加而增加;体积分数75%的乙醇对大孔吸附树脂DA201-C的洗脱效果最佳;DA201-C大孔吸附树脂对水解液脱盐的最佳条件为上样流速0.5 BV/h,上样质量浓度为30 mg/mL,水清洗速率为2 BV/h,洗脱采用体积分数75%的乙醇以2 BV/h的流速洗脱,在此条件下大孔吸附树脂DA201-C对水解液的脱盐率达97.17%。

关键词: 草鱼, 蛋白水解液, 大孔吸附树脂, 吸附, 解吸

Abstract:

In order to remove salt from grass carp protein hydrolysate, four macroporous adsorption resins including
DA201-C, DA201-M, SQT-67 and D002 were selected for the highest adsorption capacity for grass carp peptides through
static adsorption and desorption experiments. The results showed that DA201-C had the best adsorption efficiency for
grass carp peptides. Its adsorption quantity increased with increasing concentration of grass carp peptides. Seventy-five
percent ethanol revealed the best elution efficiency for DA201-C. The optimal conditions for the desalination of grass carp
peptides with DA201-C were determined as follows: the sample at 30 mg/mL was passed through the column at a flow rate
0.5 BV/h, and then the column was washed with at a flow rate of 2 BV/h and eluted with 75% ethanol at 2 BV/h. Under
these conditions, the desalination efficiency of grass carp peptides was 97.17%.

Key words: grass carp, protein hydrolysate, macroporous adsorption resin, adsorption, desorption

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