一株降解呕吐毒素蜡样芽孢杆菌的筛选与鉴定

doi:Q939.99

食品科学

一株降解呕吐毒素蜡样芽孢杆菌的筛选与鉴定

余祖华1,2，丁轲1,2,*，刘赛宝1，李亚菲2，李旺1，李元晓1，曹平华1，刘一尘2，孙二刚3

1.河南科技大学宏翔发酵饲料实验室，河南洛阳 471003；2.河南省动物疫病与公共安全院士工作站，河南洛阳 471003；3.河南宏翔生物科技有限公司，河南汝州 467500

出版日期:2016-03-15 发布日期:2016-03-17

Screening and Identification of a Bacillus cereus Strain Able to Degradate Deoxynivalenol

YU Zuhua1,2, DING Ke1,2,*, LIU Saibao1, LI Yafei2, LI Wang1, LI Yuanxiao1, CAO Pinghua1, LIU Yichen2, SUN Ergang3

1. Hongxiang Biological Feed Laboratory, Henan University of Science and Technology, Luoyang 471003, China;
2. Animal Disease and Public Safety Academician Workstation of Henan Province, Luoyang 471003, China;
3. Henan Hongxiang Biotechnology Co. Ltd., Ruzhou 467500, China

Online:2016-03-15 Published:2016-03-17

摘要/Abstract

摘要：

目的：分离筛选能够降解呕吐毒素（deoxynivalenol，DON）的芽孢杆菌，以用于该毒素的生物降解。方法：采集霉变秸秆、土壤和粪便样品，先将样品加热80 ℃后，取上清液接种到以DON为唯一碳源的分离培养基富集DON降解菌。以LB培养基分离纯化富集菌，然后对分离到的菌株进行DON毒素降解能力检测。对降解能力最强的菌株进行形态学观察、生理生化实验和16S rDNA序列鉴定。结果：从16 株分离菌中筛选得到一株对DON降解能力最强的菌株B.JG05，降解率最高可达80.61%，且对含DON饲料的降解率为82.68%。该菌株呈短杆状，能形成芽孢；生理生化特性符合蜡样芽孢杆菌的基本特征；16S rDNA序列进化树分析表明该菌株与蜡样芽孢杆菌的亲缘关系最近。结论：筛选获得了一株高效降解DON的蜡样芽孢杆菌B.JG05，为饲料和食品中DON毒素的生物降解提供了可能。

关键词: 呕吐毒素, 蜡样芽孢杆菌, 降解, 筛选, 鉴定

Abstract:

Objective: To isolate and identify a Bacillus strain used for biodegradation of deoxynivalenol (DON). Methods:
Samples of moldy straw, soil and faeces were collected and heated to 80 ℃. The supernatant was inoculated into an isolation
medium with DON as the sole carbon source to enrich DON-degrading strains. The enriched strains were isolated and
purified on LB medium plates, and then the isolates were detected for their ability to degrade DON. The optimal strain
was identified by morphological observation, physiological and biochemical tests and 16S rDNA sequences. Results:
The optimal strain B.JG05 was screened out of 16 isloates, which could degrade up to 80.61% of DON in inorganic salt
medium. The degradation efficiency of DON in feed by B.JG05 was 82.68%. The strain B.JG05 was a rod-shaped and sporeforming
bacterium. Its physiological and biochemical characteristics were consistent with those of Bacillus cereus, and the
phylogentic tree analysis based on 16S rDNA sequence showed that it was the most close to Bacillus cereus. Conclusion:
The strain B.JG05 with high DON degradation capacity was identified as Bacillus cereus, and it could offer a basis for the
biodegradation of DON in feed or food.

Key words: deoxynivalenol, Bacillus cereus, degradation, screening, identification

中图分类号:

10.7506/spkx1002-6630-201605022

余祖华，丁轲，刘赛宝，李亚菲，李旺，李元晓，曹平华，刘一尘，孙二刚. 一株降解呕吐毒素蜡样芽孢杆菌的筛选与鉴定[J]. 食品科学, doi: Q939.99.

YU Zuhua, DING Ke, LIU Saibao, LI Yafei, LI Wang, LI Yuanxiao, CAO Pinghua, LIU Yichen, SUN Ergang. Screening and Identification of a Bacillus cereus Strain Able to Degradate Deoxynivalenol[J]. FOOD SCIENCE, doi: Q939.99.

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一株降解呕吐毒素蜡样芽孢杆菌的筛选与鉴定

Screening and Identification of a Bacillus cereus Strain Able to Degradate Deoxynivalenol

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