二十碳五烯酸对THP-1巨噬细胞源性泡沫细胞形成的影响及机制

doi:10.7506/spkx1002-6630-201605029

食品科学

二十碳五烯酸对THP-1巨噬细胞源性泡沫细胞形成的影响及机制

王炎炎1，宋志秀1,2，杨立刚1，夏惠1，孙桂菊1,*

1.东南大学公共卫生学院营养与食品卫生学系，环境医学工程教育部重点实验室，江苏南京 210009；
2.南京中医药大学第二临床医学院，江苏南京 210046

出版日期:2016-03-15 发布日期:2016-03-17

Effect of Eicosapetaenoic Acid on THP-1 Macrophage-Derived Foam Cell Formation and Its Mechanism of Action

WANG Yanyan1, SONG Zhixiu1,2, YANG Ligang1, XIA Hui1, SUN Guiju1,*

1. Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China; 2. Second Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210046, China

Online:2016-03-15 Published:2016-03-17

摘要/Abstract

摘要：

目的：研究二十碳五烯酸（eicosapentaenoic acid，EPA）对THP-1巨噬细胞源性泡沫细胞形成的影响。方法：将THP-1单核细胞、佛波酯（phorbol myristate acetate，PMA）和氧化型低密度脂蛋白（oxidized low densitylipoprotein，ox-LDL）共同培养建立泡沫细胞模型，以不同浓度（200、100、50 μmol/L）的EPA处理细胞48 h后，采用酶法测定细胞内总胆固醇（total cholesterol，TC）及胆固醇酯（cholesterol ester，CE）含量，采用酶联免疫吸附实验（euzyme-linked immunosorbent assay，ELISA）测定细胞上清液中白细胞介素-6（interleukin-6，IL-6）及肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的含量，采用半定量反转录聚合酶链式反应（reverse transcriptpolymerase chain reaction，RT-PCR）测定三磷酸腺苷结合盒转运体A1（ATP binding cassette transporter A1，ABCA1）mRNA表达水平。结果：与模型组比较，EPA干预组细胞内TC及CE含量均降低，并存在显著差异（P＜0.05）；细胞上清液中IL-6及TNF-α含量也显著降低（P＜0.05），并具有量-效关系；低浓度的EPA具有上调ABCA1 mRNA表达的作用。结论：EPA能够降低泡沫细胞内TC的蓄积，抑制泡沫细胞的形成，降低IL-6及TNF-α炎性因子的分泌水平，能够上调ABCA1 mRNA表达水平，进而预防动脉粥样硬化的发生发展。

关键词: 二十碳五烯酸, 泡沫细胞, 动脉粥样硬化

Abstract:

Objective: To investigate the effect of eicosapetaenoic acid (EPA) on the formation of THP-1 monocyte-derived
foam cells. Methods: THP-1 monocytes were cultured with phorbol myristate acetate (PMA) and oxidized low-density
lipoprotein (ox-LDL), and intervened by EPA at concentrations of 200, 100 and 50 μmol/L for 48 hours. The cholesterol
content of foam cells was measured by cholesterol kit. The concentrations of IL-6 and TNF-α in culture supernatant were
detected by ELISA. The expression of ATP-binding cassette transporter A1 (ABCA1) was detected by semi-quantitative
reverse transcription-polymerase chain reaction (RT-PCR). Results: EPA decreased the intracellular concentration of
cholesterol (P < 0.05) when compared with the control group. EPA decreased the concentrations of IL-6 and TNF-α in
culture supernatant (P < 0.05) in a dose-dependent manner and low-dose EPA increased the expression of ABCA1 mRNA.
Conclusion: EPA reduces the accumulation of intracellular cholesterol and inhibits the transformation of macrophages
into foam cells. EPA decreases the secretion of inflammatory factor and low-dose of EPA increases the expression of
ABCA1 mRNA, which may partially explain its anti-atherosclerotic activity.

Key words: eicosapetaenoic acid, foam cell, atherosclerosis

中图分类号:

R151.1

王炎炎，宋志秀，杨立刚，夏惠，孙桂菊. 二十碳五烯酸对THP-1巨噬细胞源性泡沫细胞形成的影响及机制[J]. 食品科学, doi: 10.7506/spkx1002-6630-201605029.

WANG Yanyan, SONG Zhixiu, YANG Ligang, XIA Hui, SUN Guiju. Effect of Eicosapetaenoic Acid on THP-1 Macrophage-Derived Foam Cell Formation and Its Mechanism of Action[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201605029.

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二十碳五烯酸对THP-1巨噬细胞源性泡沫细胞形成的影响及机制

Effect of Eicosapetaenoic Acid on THP-1 Macrophage-Derived Foam Cell Formation and Its Mechanism of Action

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