食品科学

• 工艺技术 • 上一篇    下一篇

大白桩菇多糖的结构鉴定及清除自由基活性

包 瑛1,马 岳1,李雅双1,2,张云琴1,仵 缘1,2,刘春兰1,2,*   

  1. 1.中央民族大学生命与环境科学学院,北京 100081;
    2.北京市食品环境与健康工程技术研究中心,北京 100081
  • 出版日期:2016-03-25 发布日期:2016-03-18

Structure Identification and Free Radical Scavenging Activity of Polysaccharide from Fruit Bodies of Leucopaxillus giganteus

BAO Ying1, MA Yue1, LI Yashuang1,2, ZHANG Yunqin1, WU Yuan1,2, LIU Chunlan1,2,*   

  1. 1. College of Life and Environmental Science, Minzu University of China, Beijing 10008l, China;
    2. Beijing Engineering Research Center of Food Environment and Public Health, Beijing 10008l, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

利用水提醇沉法提取大白桩菇粗多糖,用酸性乙醇分级、SepharoseCL-4B以及Sephadex G-100柱层析分离纯化得到大白桩菇多糖(DBZG4-b)。采用高效液相色谱法检测DBZG4-b均一性及相对分子质量。经气相色谱、纸层析、红外光谱、甲基化、气相色谱-质谱、核磁共振方法解析DBZG4-b的结构。结果显示,DBZG4-b为均一性多糖,相对分子质量约为6 864;DBZG4-b为多分支结构多糖,主链由Glc和Gal构成,均以1→6糖苷键连接,Glc在3位上有分支,Gal在4位上有分支;支链由Ara、Glc、Gal构成,其中Ara以1→3连接,Glc以1→3连接,Gal以1→4连接,Ara、Xyl、Rha、Man、Glc、Gal都有一部分构成分子的末端残基。清除·OH与1,1-二苯基-2-三硝基苯肼自由基实验表明,DBZG4-b具有良好的清除自由基能力。

关键词: 大白桩菇, 多糖, 分离纯化, 结构, 自由基

Abstract:

Crude polysaccharide was extracted from the fruit bodies of Leucopaxillus giganteus by water extraction and
alcohol precipitation and was purified through sequential steps: acidic ethanol fractionation, Sepharose CL-4B and Sephadex
G-100 gel filtration chromatography to homogeneity as confirmed by high performance liquid chromatography (HPLC).
The purified polysaccharide was designated as DBZG4-b and its molecular weight was measured by HPLC. Moreover, the
structure of DBZG4-b was elucidated by means of gas chromatography, paper chromatography, infrared (IR) spectroscopy,
gas chromatography-mass spectrometry after methylation, and nuclear magnetic resonance (NMR). Results showed that the
average relative molecular weight of the homogeneous polysaccharide, DBZG4-b, was 6 864. Its main chains were composed
of Glc and Gal, and both of them were conjugated to 1→6 glycoside bond; Glc was substituted at 3-O while Gal was
substituted at 4-O. The side chain was composed of (1→3)-Ara, (1→3)-Glc, and (1→4)-Gal. The terminal residue was made
up of Ara, Xyl, Rha, Man, Glc and Gal. Furthermore, DBZG4-b had significant scavenging potential against hydrolxyl and
DPPH free radicals.

Key words: Leucopaxillus giganteus, polysaccharide, isolation and purification, structure, free radicals

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