食品科学

• 分析检测 • 上一篇    下一篇

HS-SPME-GC-MS技术分析香草兰果皮的挥发性成分

初 众1,2,王海茹1,3,张彦军1,2,姚 晶3,*   

  1. 1.中国热带农业科学院香料饮料研究所,海南 万宁 571533;
    2.农业部香辛饮料作物遗传资源利用重点实验室,国家重要热带作物工程技术研究中心,海南 万宁 571533;
    3.黑龙江东方学院食品与环境工程学部,黑龙江 哈尔滨 150086
  • 出版日期:2016-03-25 发布日期:2016-03-18

GC-MS Analysis of Volatiles in Vanilla Husk Extracted by Headspace Solid-Phase Microextraction

CHU Zhong1,2, WANG Hairu1,3, ZHANG Yanjun1,2, YAO Jing3,*   

  1. 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, China;
    2. Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, National Center of Important Tropical Crops Engineering and Technology Research, Wanning 571533, China;
    3. Department of Food and Environmental, East University of Heilongjiang, Harbin 150086, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

香草兰属兰科多年生攀缘藤本植物,为典型的热带经济作物,因其独特的香气成分而被广泛应用于多个领域。采用顶空固相微萃取-气相色谱-质谱联用技术分析香草兰果皮中挥发性成分。以萃取种类数多少及衡量萃取效果的萃取量Y值大小为指标,优化顶空固相微萃取进样条件,得出最佳萃取条件为:2 g香草兰果皮粉末,75 μm碳分子筛/聚二甲基硅氧烷萃取头、萃取温度80 ℃以及萃取时间20 min。在最佳萃取条件下萃取香草兰果皮中的挥发性成分,结果表明:从香草兰果皮中共检测出72 种化合物,其主要挥发性成分为芳香族33 种,相对含量最高占96.34%;其次为烷烃类12 种(0.66%)、酯类8 种(0.27%)、酸类7 种(0.79%)、醇类5 种(0.50%)、杂环类3 种(0.09%)、酮类2 种(0.37%)和醛类2 种(0.09%)。

关键词: 顶空固相微萃取-气相色谱-质谱联用, 香草兰果皮, 挥发性成分

Abstract:

Vanilla is a tropical orchid originating from Mexico, and the genus Vanilla belongs to the family Orchidaceae. It
is known as a spice with special aroma. Volatiles in vanilla husk were investigated by headspace solid-phase microextraction
(HS-SPME) and gas chromatography-mass spectrometry. Based on the number of extracted compounds and extraction
efficiency, the optimum HS-SPME conditions were determined as follows: 2 g of vanilla husk was extracted at 80 ℃ for 20 min
with a 75 μm carboxen/polydimethylsiloxane (CAR/PDMS) fiber. A total of 72 volatile compounds were identified from
vanilla husk, with 33 aromatic compounds (96.34%) constituting the main part of the volatile compounds. Minor components
included 12 alkanes (0.66%), 8 esters (0.27%), 7 acids (0.79%), 5 alcohols (0.50%), 3 heterocyclic (0.09%), 2 ketone (0.37%)
and 2 aldehydes (0.09%).

Key words: headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), vanilla husk, volatile components

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