食品科学

• 分析检测 • 上一篇    下一篇

岭南山竹子果实的化学成分分离与确定

黎 平1,郭志永1,谷荣辉1,张贝西1,吴晓欢1,李玉婷1,龙春林1,2,*   

  1. 1.中央民族大学生命与环境科学学院,北京 100081;2.中国科学院昆明植物研究所,云南 昆明 650201
  • 出版日期:2016-03-25 发布日期:2016-03-18

Separation and Identification of Chemical Constituents from Garcina oblongifolia Fruits

LI Ping1, GUO Zhiyong1, GU Ronghui1, ZHANG Beixi1, WU Xiaohuan1, LI Yuting1, LONG Chunlin1,2,*   

  1. 1. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
    2. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

目的:通过高效液相色谱-飞行时间质谱法对岭南山竹子果实的化学组分进行分析。方法:利用硅胶柱色谱、凝胶柱色谱、高速逆流色谱、高效液相色谱等分离和纯化化合物,并以核磁共振确定化合物的结构。结果:基于高效液相色谱-飞行时间质谱法的分析,初步鉴定了11 个化合物,主要为双黄酮类和多戊异烯基取代的苯甲酮类。提取分离得到5 个化合物,分别鉴定为:oblongifolin C(1)、oblongifolin A(2)、volkensiflavone(3)、GB-1a(4)和β-谷甾醇(5)。结论:从该植物中分离得到化合物3和化合物4,确定岭南山竹子果实的化学成分,可为开发该热带水果提供数据参考。

关键词: 岭南山竹子果实, 苯甲酮类, 双黄酮类, 高效液相色谱-飞行时间质谱

Abstract:

High performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF-MS) was
applied to analyze the chemical composition of Garcina oblongifolia fruits. The crude extract of Garcina oblongifolia fruits
was separated and purified by sequential chromatographies using silica gel column, Sephadex LH-20 column, high speed
counter current chromatograhy (HSCCC). The structures of the purified compounds were confirmed by nuclear magnetic
resonance (NMR). As a result, 11 compounds, which were mainly categorized to benzophenones and biflavonoids, were
tentatively identified from the fruit extract. Further isolation yielded 5 compounds, which were identified as oblongifolin C (1),
oblongifolin A (2), volkensiflavone (3), GB-1a (4) and β-sitosterol (5), respectively, of which compounds 3 and 4 were isolated
from this plant for the first time. This study provides important evidence supporting the exploitation of this tropical fruit.

Key words: Garcina oblongifolia fruits, benzophenones, biflavonoids, high performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF-MS)

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