食品科学

• 分析检测 • 上一篇    下一篇

液相31P-NMR快速检测鱼子酱中磷脂

张明霞1,庞建光2,呼秀智3,姬红萍4   

  1. 1.河北工程大学理学院,河北 邯郸 056038;2.河北工程大学建筑学院,河北 邯郸 056038;
    3.河北工程大学成人教育学院,河北 邯郸 056021;4.河北邯郸县农牧局,河北 邯郸 056006
  • 出版日期:2016-03-25 发布日期:2016-03-18

Determination of Phospholipids in Caviar by Solution 31P-NMR

ZHANG Mingxia1, PANG Jianguang2, HU Xiuzhi3, JI Hongping4   

  1. 1. School of Science, Hebei University of Engineering, Handan 056038, China;
    2. School of Architecture, Hebei University of Engineering, Handan 056038, China;
    3. College of Continuing Education, Hebei University of Engineering, Handan 056021, China;
    4. Agriculture and Animal Husbandry Bureau of Handan County, Handan 056006, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

建立液相31P-核磁共振光谱法快速检测鱼子酱食品中磷脂的分析方法。样品匀浆后氯仿和甲醇混合溶剂提取其中的全脂,无需分离直接溶于氘代试剂后进行核磁分析。该方法回收率为96%~102%,相对标准偏差不超过5%。采用本方法对4 种品牌鱼子酱食品检测均发现6 种磷脂组分存在,且圆鳍鱼籽中磷脂酰胆碱和溶血磷脂酰胆碱总量均占总磷脂量的80%以上,而鲟鱼籽中磷脂酰肌醇含量远高于圆鳍鱼籽。其他3 种磷脂磷脂酰乙醇胺、鞘磷脂和甘油磷脂酸在2 种取材的鱼子酱中含量相差不多。

关键词: 31P-核磁共振光谱法, 磷脂, 鱼子酱, 检测

Abstract:

An improved method for the determination of phospholipids in caviar by solution 31P-nuclear magnetic
resonance (31P-NMR) was studied. The lipids in the sample were extracted with chloroform and methanol, dissolved in
deuterated reagent and then analyzed by 31P-NMR without separation. The average recoveries of the established method
were 96%–102%, with relative standard deviation (RSD) not exceeding 5%. Phospholipids of 4 commercial brands of
caviar were analyzed and the relative distribution of phospholipid components was measured in mole percentage. It was
found that phosphatidylcholine and lysophosphatidylcholine accounted for more than 80% of the total phospholipids in
lumpfish roe, and phosphatidylinositol content in sturgeon roe was much higher than that of lumpfish roe. The contents of
phosphatidylethanolamine, sphingomyelin and phosphatidic acid were similar in both fish roes.

Key words: 31P-nuclear magnetic resonance (31P-NMR), phospholipids, caviar, determination

中图分类号: