食品科学

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采用GC×GC-TOFMS结合顶空固相微萃取技术检测枯草芽孢杆菌发酵液中吡嗪类物质

周庆伍1,汤有宏1,2,徐祥浩1,2,刘国英1,2,高江婧1,2,姜 利1   

  1. 1.安徽古井贡酒股份有限公司,安徽 亳州 236820;2.安徽省固态发酵工程技术研究中心,安徽 亳州 236820
  • 出版日期:2016-03-25 发布日期:2016-03-18

Using GC×GC-TOFMS Combined with Headspace Solid Phase Micro-Extraction to Detect Pyrazines in Bacillus subtilis Fermentation Broth

ZHOU Qingwu1, TANG Youhong1,2, XU Xianghao1,2, LIU Guoying1,2, GAO Jiangjing1,2, JIANG Li1   

  1. 1. Anhui Gujing Distillery Co. Ltd., Bozhou 236820, China;
    2. Anhui Engineering Research Center of Solid-State Fermentation, Bozhou 236820, China
  • Online:2016-03-25 Published:2016-03-18

摘要:

应用全二维气相色谱-飞行时间质谱联用仪结合顶空固相微萃取技术同时检测枯草芽孢杆菌发酵液中吡嗪类物质。结果表明:标准曲线线性关系良好,相关系数r2均大于0.99,相对标准偏差在4.2%~10.9%之间,加标回收率在84%~116%之间,满足发酵液样品中吡嗪类物质定量要求。

关键词: 全二维气相色谱-飞行时间质谱, 顶空固相微萃取, 枯草芽孢杆菌, 发酵液

Abstract:

 Pyrazines are one of the most important flavors in Chinese liquor. The pyrazines in Chinese liquor were analyzed
by comprehensive two dimensional gas chromatography-time of flight mass spectrometry combined with headspace solid
phase micro extraction. The results showed that 10 pyrazines were detected in fermentation broth samples and the standard
curves for all the analytes exhibited a good linear relationship (r2 > 0.99), with a relative standard deviation (RSD) between
4.2% and 10.9%. The average recoveries of spiked samples ranged between 84%–116%. This method can meet the
requirements for pyrazine detection in Bacillus subtilis fermentation broth.

Key words: gas chromatography-time of flight mass spectrometry (GC×GC-TOFMS), headspace solid phase micro extraction (HS-SPME), Bacillus subtilis, fermentation broth

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