食品科学

• 生物工程 • 上一篇    下一篇

以浒苔为碳源的地衣芽孢杆菌发酵产蛋白酶条件优化

王 颖1,高 翔2,周 君1,张春丹1,李 晔1,王祖忠1,袁 贝1,戴 娟1,钱琴莲1,苏秀榕1,*   

  1. 1.宁波大学海洋学院,浙江 宁波 315211;2.浙江省乔司监狱,浙江 杭州 310019
  • 出版日期:2016-04-15 发布日期:2016-04-13

Optimization of Fermentation Conditions for Protease Production by Bacillus licheniformis with Enteromorpha prolifera as Carbon Source

WANG Ying1, GAO Xiang2, ZHOU Jun1, ZHANG Chundan1, LI Ye1, WANG Zuzhong1, YUAN Bei1, DAI Juan1, QIAN Qinlian1, SU Xiurong1,*   

  1. 1. School of Marine Sciences, Ningbo University, Ningbo 315211, China; 2. Qiaosi Prison in Zhejiang, Hangzhou 310019, China
  • Online:2016-04-15 Published:2016-04-13

摘要:

为了优化地衣芽孢杆菌(Bacillus licheniformis)以浒苔(Enteromorpha prolifera)为碳源进行发酵产蛋白酶的能力,以蛋白酶比活力为指标,通过单因素试验探讨不同因素对产酶效率的影响,在此基础上运用Plackett-Burman设计筛选出了3 个显著因素:培养基初始pH值、浒苔添加量和NaH2PO4添加量。采用响应面法对这3 个显著因素进行优化,得到最佳产酶条件为培养基初始pH 6.66、浒苔添加量4.65%、NaH2PO4添加量0.027 4%,此条件下蛋白酶比活力预测值为66 354.70 U/g pro,3 次实验验证值为66 966.37 U/g pro。验证值与基础发酵条件下的最大蛋白酶比活力18 206.16 U/g pro相比,提高了2.68 倍。

关键词: 地衣芽孢杆菌, 浒苔, 发酵条件, 蛋白酶, Plackett-Burman设计

Abstract:

This work reports on the selection and optimization of culture conditions and medium components as well as their
levels for improved production of protease by Bacillus licheniformis with Enteromorpha prolifera as a carbon source by the
combined use of single factor method, Plackett-Burman design and response surface methodology (RSM). Initial medium
pH, Enteromorpha prolifera concentration and NaH2PO4 concentration were identified as the most significant factors that
influence protease production. The levels of the three factors were optimized using RSM to be 6.66, 4.65% and 0.027 4%,
respectively. Under the optimized conditions, the predicted values of protease activity was 66 354.70 U/g protein and an
average value of 66 966.37 U/g protein was obtained from three replicate experiments, which was 3.68 times higher than that
before optimization.

Key words: Bacillus licheniformis, Enteromorpha prolifera, fermentation conditions, protease, Plackett-Burman design

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