生姜中Salmonella enterica的分离与宿主氧化应答

doi:10.7506/spkx1002-6630-201607028

食品科学

生姜中Salmonella enterica的分离与宿主氧化应答

黄科1,2，李泊宁1，罗洁1,2，庞敏1，刘奕清1,2，邹勇1,2,*

1.重庆文理学院林学与生命科学学院，重庆 402160；2.重庆文理学院特色植物研究所，重庆市特色种苗工程技术研究中心，重庆高校园林花卉工程研究中心，重庆 402160

出版日期:2016-04-15 发布日期:2016-04-13

Salmonella enterica Causes Bacterial Disease in Ginger and Induces Host Antioxidant Response

HUANG Ke1,2, LI Boning1, LUO Jie1,2, PANG Min1, LIU Yiqing1,2 , ZOU Yong1,2,*

1. College of Forestry and Life Sciences, Chongqing University of Arts and Sciences, Chongqing 402160, China; 2. Chongqing Engineering Research Center for Special Plant Seedlings, Garden Flower Engineering Research Center of Chongqing Colleges, Research Institute of Special Plant, Chongqing University of Arts and Sciences, Chongqing 402160, China

Online:2016-04-15 Published:2016-04-13

摘要/Abstract

摘要：

本研究对重庆荣昌采后发病姜块进行病原菌分离鉴定，分离到一株新的生姜致病菌。通过细菌形态、生理生化、16S rDNA遗传分析鉴定为沙门氏菌（Salmonella enterica）。将沙门氏菌回接姜苗、姜块，发现姜苗叶片失绿，褶皱；姜块表面水渍并有分泌物；动态污染研究表明：沙门氏菌能在姜块中迅速繁殖，显著提高姜块超氧化物歧化酶（superoxide dismutase，SOD）、过氧化物酶（peroxidase，POD）、过氧化氢酶（catalase，CAT）的活性。该研究表明，沙门氏菌能以生姜为寄主存活。

关键词: 生姜, 分离, 沙门氏菌, 抗氧化应答

Abstract:

Ginger is an important economic crop with high medicinal and nutritional value. Diseases caused by pathogenic
bacteria in ginger have negative impacts on its growth and storage postharvest quality, and often result in significant profit
reduction. In the present study, a novel bacterial pathogen, Salmonella enterica, was isolated from postharvest rhizomes
in Rongchang, Chongiqng city. The bacterium was identified by 16S rDNA sequence analysis, and morphological,
physiological and biochemical tests. After being inoculated with S. enterica, ginger leaves were found to suffer from
chlorosis and wrinkle and water and secretion appeared on the surface of rhizomes. Analysis of the population dynamics of
S. enterica indicated that the bacterium colonized and grew well on ginger rhizomes. Biochemical analyses indicated that
S. enterica activated antioxidant enzyme activities including superoxide dismutase, peroxidase and catalase in host tissues.
These results reveal that S. enterica has the capacity to parasitize ginger.

Key words: ginger, isolate, Salmonella enterica, antioxidant response

中图分类号:

Q93.331

黄科，李泊宁，罗洁，庞敏，刘奕清，邹勇. 生姜中Salmonella enterica的分离与宿主氧化应答[J]. 食品科学, doi: 10.7506/spkx1002-6630-201607028.

HUANG Ke, LI Boning, LUO Jie, PANG Min, LIU Yiqing, ZOU Yong. Salmonella enterica Causes Bacterial Disease in Ginger and Induces Host Antioxidant Response[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201607028.

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生姜中Salmonella enterica的分离与宿主氧化应答

Salmonella enterica Causes Bacterial Disease in Ginger and Induces Host Antioxidant Response

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