低聚体葡萄籽原花青素对顺铂损伤HEK293细胞及抗癌活性的影响

doi:10.7506/spkx1002-6630-201607033

食品科学

低聚体葡萄籽原花青素对顺铂损伤HEK293细胞及抗癌活性的影响

连燕娜，高丽萍*，郭豫，高兆兰，金玉

北京联合大学应用文理学院，生物活性物质与功能食品北京市重点实验室，北京 100083

出版日期:2016-04-15 发布日期:2016-04-13

Protective Effect of Grape Seed Oligomeric Proanthocyanidins Extract against Cisplatin-Induced Nephrotoxicity in HEK293 Cell and Effect on Anticancer Activity of Cisplatin in Human Lung Cancer Cells

LIAN Yanna, GAO Liping*, GUO Yu, GAO Zhaolan, JIN Yu

Beijing Municipal Key Laboratory of Biological Active Substances and Functional Food, College of Applied Arts and Science, Beijing Union University, Beijing 100083, China

Online:2016-04-15 Published:2016-04-13

摘要/Abstract

摘要：

目的：探讨低聚体葡萄籽原花青素（grape seed proanthocyanidins extract，GSPE）及其结构单元儿茶素（catechin，C）对顺铂（cis-dichlorodiamineplatinum (II)，DDP）损伤HEK293人胚肾细胞的保护作用以及对DDP抗A549人肺腺癌细胞活性的影响。方法：体外培养HEK293细胞和A549细胞，以DDP建立损伤模型，分别用不同质量浓度的低聚体GSPE和儿茶素对细胞进行预处理，四甲基偶氮唑蓝（methyl thiazolyl tetrazolium，MTT）法测定细胞抑制率和存活率。结果：当低聚体GSPE质量浓度为16 mg/L时，对DDP诱导的HEK293细胞损伤的保护作用最佳，与DDP损伤组相比有显著差异（P＜0.05），并且在达到此质量浓度时可显著增强DDP对A549细胞的损伤效果（P＜0.05）。相同条件下儿茶素对DDP诱导的HEK293细胞损伤无显著影响（P＞0.05）。结论：体外培养条件下，一定质量浓度的低聚体GSPE对DDP诱导的HEK293细胞损伤具有一定的保护作用，同时能增强DDP对A549细胞的杀伤作用。儿茶素对DDP诱导HEK293细胞损伤的保护作用不明显。

关键词: 低聚体葡萄籽原花青素, 儿茶素, 顺铂, 肾毒性

Abstract:

Objective: To investigate the protective effect of grape seed oligomeric proanthocyanidins extract (GSOPE) and
catechin (C) against cisplatin (DDP)-induced nephrotoxicity in human embryonic kidney (HEK) 293 cells and its effect on
anticancer activity of DDP in human lung cancer A549 cells. Methods: HEK293 cells and A549 cells were cultured in vitro.
The protective effects of GSOPE and catechin at various concentrations on DDP-induced HEK293 cells and their synergistic
interaction with DDP against A549 cells were evaluated by MTT assay. Results: GSOPE at 16 mg/L could significantly
protect DDP-induced HEK239 cells from death (P < 0.05), and the protective effect was better than that of other groups.
GSOPE at 16 mg/L could also significantly enhance DDP-induced A549 cell death (P < 0.05). On the other hand, catechin
had no influence on DDP-induced HEK293 cell death. Conclusion: In vitro, GSOPE, rather than catechin, can protect
DDP-induced nephrotoxicity and can enhance DDP-induced lung cancer cell death.

Key words: grape seed oligomeric proanthocyanidins extract (GSOPE), catechin, cisplatin, nephrotoxicity

中图分类号:

Q946.8

连燕娜，高丽萍，郭豫，高兆兰，金玉. 低聚体葡萄籽原花青素对顺铂损伤HEK293细胞及抗癌活性的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201607033.

LIAN Yanna, GAO Liping, GUO Yu, GAO Zhaolan, JIN Yu. Protective Effect of Grape Seed Oligomeric Proanthocyanidins Extract against Cisplatin-Induced Nephrotoxicity in HEK293 Cell and Effect on Anticancer Activity of Cisplatin in Human Lung Cancer Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201607033.

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低聚体葡萄籽原花青素对顺铂损伤HEK293细胞及抗癌活性的影响

Protective Effect of Grape Seed Oligomeric Proanthocyanidins Extract against Cisplatin-Induced Nephrotoxicity in HEK293 Cell and Effect on Anticancer Activity of Cisplatin in Human Lung Cancer Cells

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