食品科学

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湘西香醋对秀丽隐杆线虫体内抗氧化作用

黄润庭,李宗军*,谭 雅,杜 莎,吴 硕,伍 婧   

  1. 湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室,湖南 长沙 410128
  • 出版日期:2016-04-15 发布日期:2016-04-13

In Vivo Antioxidant Activity of Xiangxi Vinegar: A Study in Caenorhabditis elegans

HUANG Runting, LI Zongjun*, TAN Ya, DU Sha, WU Shuo, WU Jing   

  1. Hunan Province Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
  • Online:2016-04-15 Published:2016-04-13

摘要:

本研究对秀丽隐杆线虫分别饲喂配制白醋、酿造白醋、发酵1 a香醋以及湘西原香醋,通过测定其体内活性氧(reactive oxygen species,ROS)含量,谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活力以及细胞凋亡相关蛋白表达情况,对湘西香醋体内抗氧化作用进行评价。结果表明:湘西香醋具有明显抗氧化效果,与对照组相比,发酵1 a香醋组与湘西原香醋组秀丽隐杆线虫体内ROS含量明显降低(P<0.01),分别为对照组的24.03%、39.44%。3 种抗氧化酶活力明显升高(P<0.01),湘西原香醋组秀丽隐杆线虫体内GSH-Px、SOD、CAT活力分别为对照组的2、1.3、3 倍。发酵1 a香醋组与湘西原香醋组秀丽隐杆线虫体内凋亡蛋白CED-3表达量明显降低,分别为对照组的53.42%、73.39%,抗凋亡蛋白CED-9表达量显著升高,分别约为对照组的7 倍和6 倍(P<0.01)。湘西香醋在秀丽隐杆线虫体内具有较强抗氧化作用。

关键词: 醋, 秀丽隐杆线虫, 活性氧, 抗氧化酶, 细胞凋亡

Abstract:

The antioxidant activity of Xiangxi vinegar was evaluated by determining the level of reactive oxygen species
(ROS), the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT), and cell
apoptosis in Caenorhabditis elegans. The results showed that Xiangxi vinegar had a remarkable antioxidant effect in
C. elegans. Compared with the control group (M9 buffer), the level of ROS in Caenorhabditis elegans treated with one-year
fermented, one-year aged Xiangxi vinegar and one-year fermented Xiangxi vinegar decreased significantly (P < 0.01) by
39.44% and 24.03%, respectively. The activities of GSH-Px, SOD and CAT in the first treatment group increased remarkably
(P < 0.01) compared with the control group, showing a 2-, 1.3- and 3-fold increase, respectively. The expression level of CED-3
in both Xiangxi vinegar treatment groups dropped significantly to 53.42% and 73.39% of the control group, respectively, whereas
the expression level of CED-9 in the treatment groups increased significantly (P < 0.01) 7 and 6 times, respectively. Therefore,
Xiangxi vinegar has strong antioxidant activity in C. elegans.

Key words: vinegar, Caenorhabditis elegans, reactive oxygen species, antioxidant enzymes, apoptosis

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