响应面试验优化真空气流细胞破壁提取蕉柑落果辛弗林工艺

doi:10.7506/spkx1002-6630-201608013

食品科学

响应面试验优化真空气流细胞破壁提取蕉柑落果辛弗林工艺

刘谋泉1，孔美兰1，刘志聪1，张福平1，陈德宾2

1.韩山师范学院生命科学与食品科技学院，广东潮州 521041；2.广东富味制果厂有限公司，广东汕头 515011

出版日期:2016-04-25 发布日期:2016-04-13

Optimization of Cell Wall Breakdown by Vacuum Air Current Technique for Synephrine Extraction from Abscising Fruits of Citrus tankan Using Response Surface Methodology

LIU Mouquan1, KONG Meilan1, LIU Zhicong1, ZHANG Fuping1, CHEN Debin2

1. College of Life Science and Food Technology, Hanshan Normal University, Chaozhou 521041, China;
2. Guangdong Fuwei Fruits & Nuts Manufacturing Co. Ltd., Shantou 515011, China

Online:2016-04-25 Published:2016-04-13

摘要/Abstract

摘要：

为开发利用蕉柑落果资源，提高蕉柑落果中辛弗林的提取率，并尽量保护蕉柑组织形态完整，对质构保护液质量浓度以及真空气流细胞破壁技术的4 个影响因素进行考察；并采用Box-Behnken试验设计及响应面分析对真空气流细胞破壁技术的工艺条件进行优化。结果表明，质构保护最佳处理工艺为：首先在质量浓度0.3 g/100 mL的海藻酸钠溶液中浸泡20 min，接着在质量浓度0.15 g/100 mL的氯化钙溶液中浸泡60 min；真空气流细胞破壁前处理最佳工艺为：泄压温度100 ℃、压力差116 kPa、停滞时间21 min、泄压3 次。此时辛弗林提取率为69.42%，破果率为33.21%；相比直接进行亚临界水提取对照组，破果率下降了12.07%，辛弗林提取率增加了26.94%。

关键词: 蕉柑, 生理落果, 真空气流细胞破壁, 辛弗林

Abstract:

In order to improve the extraction efficiency of synephrine from abscising fruits of Citrus tankan and
simultaneously protect the morphological integrity of tissues, vacuum air current technique was applied in cell wall
breakdown for subcritical water extraction of synephrine. The effect of two tissue protectants on the percentage of broken
fruits and extraction efficiency was examined. The experimental conditions for cell wall breakdown were optimized to
maximize the extraction efficiency of synephrine by Box-Behnken design and response surface methodology (RSM) based
on single-factor experiments. Sequential soaking in 0.3 g/100 mL sodium alginate solution for 20 min and 0.15 g/100 mL
calcium chloride solution for another 60 min provided optimal protection of the tissue. The optimum conditions for cell wall
breakdown were determined as follows: decompression temperature, 100 ℃; pressure difference, 116 kPa; retention time,
21 min; and 3 decompression cycles. Under these pretreatment conditions, the extraction yield of synephrine was 69.42%
and the percentage of broken fruits was 33.21%, which were increased by 26.94% and decreased by 12.07% compared with
the control group without this pretreatment, respectively.

Key words: Citrus tankan, abscising fruits, vacuum air current for plant cell wall breakdown (VAPB), synephine

中图分类号:

TS255.1

刘谋泉，孔美兰，刘志聪，张福平，陈德宾. 响应面试验优化真空气流细胞破壁提取蕉柑落果辛弗林工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201608013.

LIU Mouquan, KONG Meilan, LIU Zhicong, ZHANG Fuping, CHEN Debin. Optimization of Cell Wall Breakdown by Vacuum Air Current Technique for Synephrine Extraction from Abscising Fruits of Citrus tankan Using Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201608013.

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响应面试验优化真空气流细胞破壁提取蕉柑落果辛弗林工艺

Optimization of Cell Wall Breakdown by Vacuum Air Current Technique for Synephrine Extraction from Abscising Fruits of Citrus tankan Using Response Surface Methodology

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