食品科学

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石榴皮木醋液的化学成分及抗氧化活性

张立华,孙凤杰,赵文亚,郑丹丹,王京龙   

  1. 枣庄学院生命科学学院,山东 枣庄 277160
  • 出版日期:2016-04-25 发布日期:2016-04-13

Chemical Constituents and Antioxidant Activities of Wood Vinegar from Pomegranate Husk

ZHANG Lihua, SUN Fengjie, ZHAO Wenya, ZHENG Dandan, WANG Jinglong   

  1. College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China
  • Online:2016-04-25 Published:2016-04-13

摘要:

为探索石榴皮资源利用的新途径,以石榴皮为原料经干馏(350~500 ℃)制备了木醋液,并采用气相色谱-质谱联用仪分析其化学成分,通过对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和羟自由基清除实验评价其抗氧化活性。结果显示,石榴皮木醋液的总酚、总酮和有机酸含量分别为10.92、7.81 mg/mL和2.53%;共检测出27 种有机化合物,包括酚类、有机酸、醇类、烷烃、醛类及酮类化合物等。石榴皮木醋液对DPPH自由基和羟自由基清除力的VC当量分别为10.22 μg/mg和45.19 μg/mg。结果表明,石榴皮木醋液可作为一种天然抗氧化剂资源,具有一定的开发利用价值。

关键词: 石榴皮, 木醋液, 化学成分, 抗氧化活性

Abstract:

In order to explore a new approach to the utilization of pomegranate husk, wood vinegar was prepared by
pyrolyzing pomegranate husk in the temperature range of 150–400 ℃. Chemical constituents of the wood vinegar were
analyzed by gas chromatography-mass spectrometry (GC-MS). Its antioxidant activities were evaluated by 1,1-diphenyl-
2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging methods. The results showed that the contents of total
phenols, total ketones and organic acid in the wood vinegar were 10.92 mg/mL, 7.81 mg/mL and 2.53% respectively. A
total of 27 compounds were detected by GC-MS analysis from the wood vinegar, mainly including phenols, organic acids,
alcohols, alkanes, aldehydes and ketones. Its scavenging activities against DPPH and OH hydroxyl radicals were 10.22 μg/mg
and 45.19 μg/mg VC equivalent, respectively. This study suggested that wood vinegar from pomegranate husk has the
potential to be used as a natural antioxidant resource.

Key words: pomegranate husk, wood vinegar, chemical constituents, antioxidant activity

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