食品科学

• 生物工程 • 上一篇    下一篇

红豆越橘酿酒优良降酸酵母的筛选及分离鉴定

杨 华,刘亚娜,郭德军*   

  1. 黑龙江八一农垦大学食品学院,黑龙江 大庆 163319
  • 出版日期:2016-05-15 发布日期:2016-05-18

Screening, Isolation and Identification of Acid-Degrading Yeast from Vaccinium vitis-idaea Wine

YANG Hua, LIU Yana, GUO Dejun*   

  1. School of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Online:2016-05-15 Published:2016-05-18

摘要:

以红豆越橘果汁为原料,在24 ℃的恒温条件下自然发酵,从中筛选出一株优良的降酸菌株,对其进行分离鉴定,并研究其降酸性能。结果表明,菌株BY-9降酸性能最好,适合对红豆越橘果酒进行生物降酸处理。对菌株BY-9进行形态学和生理学特征鉴定以及内源转录间隔区(internally transcribed spacer,ITS)序列解析,鉴定出菌株BY-9为酵母属中的二孢结合酵母(Zygosaccharomyces bisporus)。将菌株BY-9接入酒精发酵结束的红豆越橘果酒中,发现其使红豆越橘果酒总酸含量降低了12.26%。

关键词: 红豆越橘, 降酸酵母, 筛选, 分离, 鉴定

Abstract:

A yeast strain with high acid-degrading ability from Vaccinium vitis-idaea juice fermented in a
a thermostatically controlled incubator at 24 ℃ was isolated and identified. Its acid degradation capacity was investigated.
The results indicated that strain BY-9, which had the highest acid-degrading capacity, was found to be the most suitable for
reducing acidity of Vaccinium vitis-idaea wine. Based on morphological and physiological characterization, and internally
transcribed spacer (ITS) analysis, strain BY-9 was identified as Zygosaccharomyces bisporus. The total acid content of
Vaccinium vitis-idaea wine was reduced by 12.26% when BY-9 was inoculated after alcoholic fermentation.

Key words: Vaccinium vitis-idaea, acid-degrading yeast, selection, isolation, identification

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