食品科学

• 工艺技术 • 上一篇    下一篇

红树莓多酚的醇法提取工艺优化

旷 慧1,迟 超1,吕长山2,王 萍1,赵 鑫1,王金玲1,*   

  1. 1.东北林业大学林学院,黑龙江 哈尔滨 150040;2.东北农业大学应用技术学院,黑龙江 哈尔滨 150030
  • 出版日期:2016-05-25 发布日期:2016-05-18

Optimization of Ethanol Extraction Process for Polyphenols from Red Raspberry

KUANG Hui1, CHI Chao1, LÜ Changshan2, WANG Ping1, ZHAO Xin1, WANG Jinling1,*   

  1. 1. School of Forestry, Northeast Forestry University, Harbin 150040, China;
    2. School of Applied Technology, Northeast Agricultural University, Harbin 150030, China
  • Online:2016-05-25 Published:2016-05-18

摘要:

以‘菲尔杜德’、‘宝石红’红树莓果实为原料,采用醇法提取红树莓多酚。以乙醇为提取溶剂,以乙醇体积分数、提取温度、料液比、提取时间、提取次数为因素,以多酚提取量为指标,通过单因素试验及L9(34)正交试验优化醇法提取红树莓多酚的工艺参数,同时采用邓肯多重范围检验法检验不同处理间的差异显著性。结果表明,‘菲尔杜德’红树莓多酚的最佳提取工艺参数为乙醇体积分数70%(pH 6)、提取温度45 ℃、料液比1∶6(g/mL)、提取时间3.5 h、提取2 次;‘宝石红’红树莓多酚的最佳提取工艺参数为乙醇体积分数65%(pH 6)、提取温度45 ℃、料液比1∶5(g/mL)、提取时间3.5 h、提取2 次。在此条件下,两种红树莓多酚提取量分别为(373.78±4.08)、(287.08±4.91)mg/100 g。该工艺可靠、稳定,具有一定的参考价值。

关键词: 红树莓, 多酚, 提取, 正交试验, 邓肯多重范围检验法

Abstract:

Fruits from two different cultivars of red raspberry, ‘Fertod Zamatos’ and ‘U.S. Raspberry’, were selected for
extracting polyphenols using ethanol as the solvent. Optimization of extraction parameters was carried out using onefactor-
at-a-time method and an orthogonal array design L9 (34). Ethanol concentration, extraction temperature, solid-tosolvent
ratio, extraction time and number of extraction cycles were chosen as the independent variables. The response was
the extraction yield of polyphenols. Duncan’s Mutiple-Range test was adopted to examine the significance of differences
among various treatments. The optimal extraction conditions for polyphenols were established as follows: 70% (V/V) ethanol
(pH 6), 45 ℃, solid-to-solvent ratio of 1:6 (g/mL), 2 extraction cycles, and 3.5 h each cycle for ‘Fertod Zamatos’; and 65%
(V/V) ethanol (pH 6), 45 ℃, solid-to-solvent ratio of 1:5 (g/mL), 2 extraction cycles, and 3.5 h each cycle for ‘U.S.
Raspberry’. The corresponding yields of polyphenols from these two cultivars were (373.78 ± 4.08) mg/100 g and (287.08 ±
4.91) mg/100 g, respectively. These results indicated that the extraction procedure was reliable, stable and practical.

Key words: red raspberry, polyphenols, extraction, orthogonal array test, Duncan’s mutiple-range test

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