食品科学

• 工艺技术 • 上一篇    下一篇

响应面试验优化纤维素酶辅助提取莲子钻芯粉中甲基莲心碱工艺

任 美1,夏延斌1,*,王亮亮2   

  1. 1.湖南农业大学食品科技学院,湖南 长沙 410128;2.湖南省食品质量监督检验研究院,湖南 长沙 410111
  • 出版日期:2016-05-25 发布日期:2016-05-18

Applying Response Surface Methodology to Optimize the Extraction of Neferine from Lotus Seed Core Powder Using Cellulase

REN Mei1, XIA Yanbin1,*, WANG Liangliang2   

  1. 1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Institute of Food Quality Supervision Inspection and Research, Changsha 410111, China
  • Online:2016-05-25 Published:2016-05-18

摘要:

以莲子钻芯粉为原料,采用纤维素酶辅助提取莲子钻芯粉中的甲基莲心碱,在单因素试验的基础上,选择加酶量、酶解温度、酶解时间、酶解pH值为自变量,以甲基莲心碱提取量为响应值,通过Box-Behnken响应面设计优选最佳工艺条件。结果表明:采用纤维素酶辅助提取莲子钻芯粉中甲基莲心碱,最佳提取工艺条件为加酶量3.1%(质量分数)、酶解时间1.7 h、酶解pH 4.8、酶解温度50 ℃。该工艺条件下得到的甲基莲心碱提取量为(7.02±0.22)mg/g,高于传统乙醇法(4.03±0.17)mg/g,提取量提高了71.9%。采用纤维素酶辅助提取法操作简单,条件温和,有效地提高了莲子钻芯粉的利用率。

关键词: 莲子钻芯粉, 甲基莲心碱, 纤维素酶, 响应面

Abstract:

The cellulase-assisted extraction of neferine from lotus seed core powder was optimized using single-factor
experiments and response surface methodology based on Box-Behnken design. The independent variables were cellulase
dosage, hydrolysis pH, temperature and time. Neferine yield was used as the response. The extraction conditions were optimized
as follows: cellulase dosage, 3.1% (m/m); pH, 4.8; hydrolysis time, 1.7 h min; and hydrolysis temperature, 50 ℃. Under the
optimized conditions, the yield of neferine was (7.02 ± 0.22) mg/g, which was 71.9% higher than that ((4.03 ± 0.17) mg/g)
obtained with the traditional ethanol extraction method. The cellulase-assisted extraction process was accomplished under
simple and mild conditions, which could effectively improve the utilization of lotus seed core powder.

Key words: lotus seed core powder, neferine, cellulas, response surface methodology

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