食品科学

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葡聚糖延缓草鱼肌原纤维蛋白冷冻变性的机理分析

鲁耀彬1,2,熊光权1,李 新1,吴文锦1,乔 宇1,丁安子1,廖 李1,王 俊1,汪 兰1,*   

  1. 1.湖北省农业科学院农产品加工与核农技术研究所,湖北省农业科技创新中心农产品加工研究分中心,湖北 武汉 430064;
    2.武汉轻工大学食品科学与工程学院,湖北 武汉 430023
  • 出版日期:2016-05-25 发布日期:2016-05-18

Cryoprotective Effect and Mechanism of Dextran with Different Molecular Weights on Denaturation of Myofibrillar Protein from Grass Carp (Ctenopharyngodon idella)

LU Yaobin1,2, XIONG Guangquan1, LI Xin1, WU Wenjin1, QIAO Yu1, DING Anzi1, LIAO Li1, WANG Jun1, WANG Lan1,*   

  1. 1. Farm Products Processing Research Sub-Center, Hubei Innovation Center of Agriculture Science and Technology,
    Institute of Agricultural Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences,
    Wuhan 430064, China; 2. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
  • Online:2016-05-25 Published:2016-05-18

摘要:

以草鱼肌原纤维蛋白为研究对象,通过研究盐溶性蛋白质量浓度、Ca2+-ATPase活性、表面疏水性、总巯基和活性巯基含量在冷冻贮藏条件下的变化,比较不同分子质量葡聚糖(添加量0.5%)和传统商业抗冻剂(4%蔗糖+4%山梨糖醇)对冻藏过程中肌原纤维蛋白的冷冻保护效果。结果表明,在冻藏过程中,葡聚糖能有效延缓草鱼肌原纤维蛋白的冷冻变性,效果优于传统商业抗冻剂;分子质量小的葡聚糖(T7)对Ca2+-ATPase活性和活性巯基的保护作用要整体优于分子质量大的葡聚糖(T20),冻藏超过15 d后延缓表面疏水性的上升和总巯基的保护上的效果也显著优于后者;因此,在工业中选用分子质量小的葡聚糖作为淡水鱼及相关制品的冷冻保护剂效果更佳。

关键词: 草鱼, 肌原纤维蛋白, 葡聚糖, 分子质量, 冷冻变性

Abstract:

The present study investigated the cryoprotective effect of dextran with different molecular weights on
denaturation of myofibrillar protein from grass carp (Ctenopharyngodon idella). The content of salt soluble protein,
Ca2+-ATPase activity, surface hydrophobicity, total sulfhydryl group and active sulfhydryl group were evaluated. Results
showed that the denaturation of grass carp myofibrillar protein was significantly delayed by dextran during frozen storage,
and the effect of dextran (0.5%) was better than that of commercial antifreeze (4% sucrose + 4% D-sorbitol). Low molecular
weight dextran (T7) was better than high molecular weight dextran (T20) in protecting the Ca2+-ATPase activity and active
sulfhydryl group and also in delaying the increase of surface hydrophobicity and the decrease of sulfhydryl group after
15 d frozen storage. Thus, the low molecular weight dextran is recommended to be used in the fishery industry as a
promising cryoprotectant.

Key words: grass carp, myofibrillar protein, dextran, molecular weight, frozen denaturation

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