食品科学

• 生物工程 • 上一篇    下一篇

两株土壤放线菌ZSM-1和ZSM-2的分离与鉴定

孔望君,蒋会芳,冉火苗,王 傲,吕 曼,杨 柯,辛志宏*   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2016-06-15 发布日期:2016-06-27

Isolation and Identification of Two Soil Actinomycetes ZSM-1 and ZSM-2

KONG Wangjun, JIANG Huifang, RAN Huomiao, WANG Ao, LÜ Man, YANG Ke, XIN Zhihong*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2016-06-15 Published:2016-06-27

摘要:

对分离自土壤的2 株编号为ZSM-1和ZSM-2的放线菌进行分类鉴定,提取基因组脱氧核糖核酸(deoxyribonucleic acid,DNA)后经聚合酶链式反应(polymerase chain reaction,PCR)扩增菌株16S rDNA,基因测序并进行同源性分析,构建进化树并进行系统发育分析,结合培养特征、生理生化特征,将该2 株菌分别鉴定为纳士维尔链霉菌(Streptomyces nashvillensis)与土地戈登氏菌(Gordonia terrae)。

关键词: 放线菌, 鉴定, 16S rDNA, 系统发育分析

Abstract:

Actinomycete strains ZSM-1 and ZSM-2 isolated from soil were studied for their taxonomy. The 16S rDNA was
amplified by polymerase chain reaction (PCR) after the extraction of mycelial genomic DNA and the amplification products
were subjected to sequencing and homology analysis. A phylogenetic tree was established based on the sequences of
16S rDNA, and cultural characteristics, physiological and biochemical properties were evaluated as well. As a result, both
strains were identified as Streptomyces nashvillensis and Gordonia terrae, respectively.

Key words: Actinomycete, identification, 16S rDNA gene, phylogenetic analysis

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