食品科学

• 营养卫生 • 上一篇    下一篇

甘蔗醋发酵产物在体外及模拟人体肠胃环境中的抗氧化活性

郑 平1,吴幼茹1,杨继伟1,邹 毅2,王在谦3,李 楠1,*   

  1. 1.广西大学生命科学与技术学院,广西 南宁 530004;2.广西大学糖业工程技术研究中心,广西 南宁 530004;
    3.广西海铭威酿酒股份有限公司,广西 来宾 546500
  • 出版日期:2016-06-15 发布日期:2016-06-27

Antioxidant Activity of Sugarcane Vinegar and Intermediate Products in Vitro and under Simulated Human Gastrointestinal Conditions

ZHENG Ping1, WU Youru1, YANG Jiwei1, ZOU Yi2, WANG Zaiqian3, LI Nan1,*   

  1. 1. College of Life Science and Technology, Guangxi University, Nanning 530004, China; 2. Center for Sugar Technology Research,
    Guangxi University, Nanning 530004, China; 3. Guangxi Haimingwei Brewing Co. Ltd., Laibin 546500, China
  • Online:2016-06-15 Published:2016-06-27

摘要:

甘蔗中含有丰富的多酚和黄酮类物质,本研究以总酚含量、黄酮含量、清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基能力、清除羟自由基(·OH)能力、总还原能力为指标,探讨由甘蔗汁发酵而成的甘蔗酒、甘蔗醋在体外及模拟人体消化环境整个过程中抗氧化活性的变化。结果表明,在酒精发酵阶段酒精对多酚和黄酮具有一定的提取作用;在醋酸发酵阶段多酚和黄酮含量呈现波动状态,这可能和发酵过程中的氧化、水解作用有关。甘蔗醋发酵产物在体外具有极强的清除DPPH自由基能力,同时甘蔗酒还具有较强清除·OH的能力。多酚和黄酮经过模拟人体肠胃消化环境后发生了一系列反应:多酚和黄酮在胃液环境中的含量极显著下降(P<0.01),而在肠液环境中含量略有上升。通过对不同发酵产物在体外及模拟人体肠胃消化环境下清除DPPH自由基能力、清除·OH能力、总还原能力的比较,表明人体肠胃环境中的抗氧化活性不仅受食物中抗氧化物质的影响,而且受消化液中pH值、蛋白酶、磷酸盐等多种生理因素的影响。

关键词: 甘蔗汁, 甘蔗酒, 甘蔗醋, 多酚, 黄酮, 模拟肠胃环境, 抗氧化活性

Abstract:

Sugarcane contains abundant polyphenols and flavonoids. In the present study, total polyphenols content,
flavonoids content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, hydroxyl radical scavenging capacity
and total reducing power were used as the indicators to evaluate the antioxidant activity of sugarcane juice, and the
corresponding wine and vinegar in vitro and after simulated human gastrointestinal digestion. Results showed that at the
alcohol fermentation alcohol extractable polyphenols and flavonoids were detected. At the acetic acid fermentation stage,
the contents of polyphenols and flavonoids presented some volatility. This may be related to the occurrence of oxidation
and hydrolysis during the fermentation process. The sugar cane vinegar possessed strong DPPH radical scavenging capacity
in vitro, and the sugarcane wine had strong hydroxyl radical scavenging capacity. A series of reactions of polyphenols and
flavonoids after simulated gastrointestinal digestion took place, and as a result, the contents of both compounds dropped very
significantly (P < 0.01) in simulated gastric environment, but increased slightly in simulated intestinal environment. The
antioxidant activity of the beverages in the human gastrointestinal environment is influenced not only by their antioxidant
substances, but also by pH, protease, phosphate and other physiological factors in the digestive juice.

Key words: sugarcane juice, sugarcane wine, sugarcane vinegar, polyphenols, flavonoids, simulated gastrointestinal environment, antioxidant activity

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