食品科学

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环介导等温扩增技术在转基因成分检测中的应用

冀国桢1,2,李 刚1,赵建宁1,杨殿林1,修伟明1,2,*   

  1. 1.农业部环境保护科研监测所,农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室/天津市农田生态
    与环境修复技术工程中心,天津 300191;2.沈阳农业大学植物保护学院,辽宁 沈阳 110866
  • 出版日期:2016-06-15 发布日期:2016-06-27

Application of Loop-Mediated Isothermal Amplification in the Detection of Genetically Modified Components

JI Guozhen1,2, LI Gang1, ZHAO Jianning1, YANG Dianlin1, XIU Weiming1,2,*   

  1. 1. Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture/Tianjin Key Laboratory of Agro-Environment and
    Agro-Product Safety/Tianjin Engineering Research Center of Agricultural Ecological & Environmental Remediation, Agro-Environmental
    Protection Institute, Ministry of Agriculture, Tianjin 300191, China; 2. College of Plant Protection, Shenyang Agricultural University,
    Shenyang 110866, China
  • Online:2016-06-15 Published:2016-06-27

摘要:

随着全球转基因作物商业化种植面积持续增长,转基因成分检测作为转基因作物安全管理的重要技术支撑受到越来越多的关注,世界各国与地区不断致力于转基因成分检测技术的开发。环介导等温扩增(loop-mediatedisothermal amplification,LAMP)技术由于简单快速等特点,近年来在转基因检测领域备受青睐。本文对LAMP技术在转基因成分检测中的最新研究与应用及其发展前景加以综述。

关键词: 环介导等温扩增, 转基因成分, 检测

Abstract:

As the global area of transgenic crops grows continually, special concerns have been raised on the detection of
genetically modified components which is regarded as important technical supports for the safety management of genetically
modified organisms (GMO). A series of detection techniques for GMOs have been developed in various countries and
regions worldwide. Recently, due to the advantages of fast and convenient operation, loop-mediated isothermal amplification
(LAMP) has been widely used in the detection of genetic modification. The present paper tries to review the latest research
and application of LAMP assays in the detection of genetically modified components.

Key words: loop-mediated isothermal amplification (LAMP), genetically modified components, detection

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