食品科学

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高效液相色谱-紫外双波长同时测定咖啡及咖啡制品中10 种化合物含量

邵金良,杨东顺,樊建麟,刘兴勇,杜丽娟,王 丽,刘宏程,汪禄祥   

  1. 1.云南省农业科学院质量标准与检测技术研究所,云南 昆明 650223;
    2.农业部农产品质量安全风险评估实验室(昆明),云南 昆明 650223
  • 出版日期:2016-06-25 发布日期:2016-06-29
  • 通讯作者: 刘宏程,汪禄祥
  • 基金资助:

    农业部2014年农业行业标准制定和修订项目;云南省科技惠民专项(农业)重点项目(2014RA054);
    云南省科技创新平台建设计划(公共科技服务)项目(2014DA001);
    云南省农业科学院农产品质量与食品安全省创新团队计划项目(2015HC025)

Simultaneous Determination of 10 Polyphenolic and Alkaloidal Components in Coffee and Coffee-Based Products by HPLC-Double Wavelength UV Detection

SHAO Jinliang, YANG Dongshun, FAN Jianlin, LIU Xingyong1, DU Lijuan, WANG Li, LIU Hongcheng, WANG Luxiang   

  1. 1. Institute of Agriculture Quality Standards and Testing Technique, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;
    2. Laboratory of Quality and Safety Risk Assessment for Agro-Products (Kunming), Ministry of Agriculture, Kunming 650223, China
  • Online:2016-06-25 Published:2016-06-29
  • Contact: LIU Hongcheng, WANG Luxiang

摘要:

建立高效液相色谱-紫外双波长测定咖啡及咖啡制品中葫芦巴碱、5-咖啡酰奎宁酸、咖啡因、绿原酸、4-咖啡酰奎宁酸、1,3-二咖啡酰奎宁酸、1,5-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸含量的方法。样品用甲醇-0.10%磷酸溶液(50∶50,V/V)作为提取溶剂经超声波提取,过聚四氟乙烯滤膜后在Shiseido CAPCELL PAK MGⅡC18色谱柱(4.6 mm×250 mm,5.0 μm)上,以甲醇-0.10%磷酸溶液为流动相进行梯度洗脱,选择254 nm和320 nm双波长进行目标化合物的检测。10 种化合物在0.10~100.0 mg/L范围内具有良好的线性关系,相关系数(r)不小于0.999,检出限为0.05~0.10 mg/kg,定量限为0.16~0.28 mg/kg。在20、40、100 mg/kg添加量的加标回收率为80.00%~113.8%,相对标准偏差在3.63%~9.10%之间。该方法操作简单、重复性和稳定性好,适合咖啡及咖啡制品中10 种化合物同时测定及定量分析。

 

关键词: 高效液相色谱, 紫外双波长, 咖啡及咖啡制品, 多酚类化合物, 生物碱类化合物

Abstract:

The aim of this study was to establish a method for the simultaneous determination of polyphenolic compounds
including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, cynarin, 1,5-dicaffeoylquinic acid, isochlorogenic
acid B, isochlorogenic acid A, isochlorogenic acid C and alkaloidal compounds including caffeine and trigonelline in
coffee and coffee-based products. These 10 components were extracted ultrasonically with 0.10% phosphoric acid solutionmethanol
(50:50, V/V) and then analyzed by high performance eliquid chromatography (HPLC) at 254 nm and 320 nm. The
chromatographic separation was performed on a Shiseido CAPCELL PAK MGⅡC18(4.6 mm × 250 mm, 5.0 μm) by
using 0.10% phosphoric acid solution-methanol as the mobile phase. The average recoveries of the 10 analytes in spiked
samples ranged from 80.00% to 113.8%, with relative standard deviations (RSDs) of 3.63%–9.10%. The calibration curves
for these analytes were linear in the range of 0.10–100.0 mg/L with correlation coefficients of more than 0.999. The limits
of detection (LODs) varied from 0.05 to 1.0 mg/kg and the limits of quantitation (LOQ) ranged from 0.16 to 0.28 mg/kg for
10 polyphenolic and alkaloidal components. The method showed good repeatability, accuracy and stability and enabled the
accurate quantification of 10 polyphenolic and alkaloidal components in coffee and coffee-based products.

Key words: HPLC, double UV wavelengths, coffee and coffee-based products, polyphenolic compounds, alkaloidal compounds

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