金蝉花多糖的抗氧化活性及结构分析

doi:10.7506/spkx1002-6630-201613004

摘要/Abstract

摘要：

本实验研究金蝉花多糖的抗氧化活性，并对其纯化组分进行结构分析。采用热水浸提、不同浓度乙醇分级沉淀的方法从金蝉花中提取多糖，分别获得50%醇沉金蝉花多糖（50% polysaccharides from Cordyceps cicadas，CP50）和80%醇沉金蝉花多糖（CP80），并检测两者的体外抗氧化活性。结果表明：CP50较CP80表现出较强的清除自由基的能力，且具有一定的还原能力和总抗氧化能力。CP50进一步经二乙氨乙基（diethylaminoethyl，DEAE）纤维素-52和Sephadex G-100凝胶柱分离纯化，得到活性多糖CPA-1和CPB-1。经紫外扫描光谱法和高效凝胶过滤色谱法鉴定CPA-1和CPB-1为均一多糖；单糖组成分析显示两个组分中均含有葡萄糖、甘露糖和半乳糖，其物质的量比分别为：1∶0.48∶0.52和1∶0.14∶0.114。红外光谱（infrared spectroscopy，IR）及刚果红实验发现，CPA-1和CPB-1具有典型的多糖红外吸收，且含有三股螺旋分子结构。

关键词: 金蝉花多糖, 抗氧化活性, 分离纯化, 结构分析

Abstract:

In this study, the antioxidant activities and structural characteristics of polysaccharides from Cordyceps cicadae
were determined. Two crude polysaccharide fractions (CP50 and CP80) were extracted from Cordyceps cicadae with hot
water and isolated by precipitation with different concentrations of ethanol. Their antioxidant activities were also analyzed.
The results indicated that both CP50 and CP80 were effective in antioxidant activity. The radical scavenging activity of
CP50 was higher than that of CP80. CP50 also showed higher reducing capacity and total antioxidant activity. CP50 was then
further purified by gel filtration on diethylaminoethyl (DEAE)-cellulose-52 and Sephadex G-100 columns, and two purified
fractions were obtained, namely CPA-1 and CPB-1. Ultraviolet spectroscopy and high-performance liquid chromatography
(HPLC) analysis revealed that CPA-1 and CPB-1 were homogeneous. Monosaccharide analysis revealed that both CPA-1
and CPB-1 were composed of Glc, Gal and Man with a molar ratio of 1:0.48:0.52 and 1:0.14:0.114, respectively. Moreover,
both polysaccharide fractions had three helix structures.

Key words: Cordyceps cicadaes polysaccharides, antioxidant activity, separation and purification, structural analysis

中图分类号:

R284.1

封燕，贡小辉，韦德群，刘英坤，赵明，于小凤，OPEYEMI Joshua Olatunji，焦心怡，欧阳臻. 金蝉花多糖的抗氧化活性及结构分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201613004.

FENG Yan, GONG Xiaohui, WEI Dequn, LIU Yingkun, ZHAO Ming, YU Xiaofeng, OPEYEMI Joshua Olatunji,JIAO Xinyi, OUYANG Zhen. Antioxidant Activity and Preliminary Structure Analysis of Polysaccharides from Cordyceps cicadas[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613004.

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