食品科学

• 基础研究 • 上一篇    下一篇

蜂胶醇提物脂质体制备及表征

郭夏丽,蓝雅惠,邹艺红,李 熊,罗丽萍   

  1. 南昌大学生命科学学院,江西 南昌 330031  
  • 出版日期:2016-07-15 发布日期:2016-07-26
  • 通讯作者: 罗丽萍
  • 基金资助:

    “十二五”农村领域国家科技计划课题(2012BDA29B01-3);江西省科技支撑计划项目/江西省国际合作
    项目(20121BDH80020)

Preparation and Characterization of Ethanol Extract of Propolis Liposomes

GUO Xiali, LAN Yahui, ZOU Yihong, LI Xiong, LUO Liping   

  1. School of Life Sciences, Nanchang University, Nanchang 330031, China
  • Online:2016-07-15 Published:2016-07-26
  • Contact: LUO Liping

摘要:

以大豆卵磷脂和胆固醇为膜材,采用薄膜分散-动态高压技术制备蜂胶醇提物(ethanol extract of propolis,EEP)脂质体,通过粒度分析仪、透射电子显微镜、清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、低温贮存实验等进行性状表征。结果表明:制备的蜂胶脂质体为大小均匀的球形,粒径为(78±14) nm,包埋率和稳定性分别达到(97.80±5.21)%和(2.53±0.05)%。4 ℃条件下保存90 d和180 d后,包埋率分别下降了16.74%和28.77%,分散系数分别增加了6.61%和14.32%,DPPH自由基清除指数分别为2.78和2.71;高效液相色谱法(high performance liquid chromatography,HPLC)分析贮存180 d后EEP脂质体中7 种物质变化分别为1.08%~6.63%,低于EEP的5.55%~19.42%,说明脂质体对EEP中的活性物质具有一定的保护作用。

关键词: 蜂胶醇提物, 脂质体, 表征, 稳定性

Abstract:

The thin film evaporation-dynamic high pressure microfluidization method was used to prepare ethanol extract
of propolis (EEP) liposomes with soybean lecithin and cholesterol as membrane materials. The characterization of EEP
liposomes was examined by dynamic light scattering particle size analyzer, transmission electron microscopy, DPPH radical
scavenging activity assay, and low temperature storage test. The results showed that the average size of the liposomes
obtained, evenly spherical in appearance, was (78 ± 14) nm, the encapsulation efficiency (97.80 ± 5.21)%, and the stability
parameter Ke value (2.53 ± 0.05)%. After the liposomes were incubated at 4 ℃ for 90 and 180 days, their encapsulation
efficiency was reduced by 16.74% and 28.77%, along with a simultaneous increase in polydispersity index (PDI) of 6.61%
and 14.32%, respectively. Moreover, antioxidant activity index (AAI) values for DPPH radical scavenging activity of the
stored liposomes were 2.78 and 2.71, respectively. The contents of 7 compounds in the liposomes changed by 1.08%–
6.63% after 180 days storage as revealed by HPLC analysis compared to 5.55%–19.42% in EEP. This study highlights the
protection of liposome on EEP.

Key words: ethanol extract of propolis, liposome, characterization, stability

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