不同温度下腐败希瓦氏菌（Shewanella putrefaciens）生长动力学模型的比较与评价

doi:10.7506/spkx1002-6630-201613026

摘要/Abstract

摘要：

以冷藏大黄鱼特定腐败菌腐败希瓦氏菌（Shewanella putrefaciens）为研究对象，采用修正Gompertz、修正Logistic和Baranyi方程拟合5、8、15 ℃和25 ℃条件下其在胰蛋白胨大豆肉汤中的生长动力学模型，采用Belehradek方程建立二级模型，探讨温度对腐败希瓦氏菌生长动力学的影响，并对模型的拟合优度及适用性进行评价。结果表明：温度对腐败希瓦氏菌生长动力学影响显著，其在5 ℃环境中延滞期较长，生长趋势得到明显抑制，当温度上升到25 ℃时，腐败希瓦氏菌的延滞期显著缩短，比生长速率随着温度的升高而增大，温度与延滞期及比生长速率均存在线性关系。采用均方根误差（root mean square error，RMSE）、残差平方和（residual sumof squares，RSS）、偏差度（bias factor，BF）、准确度（accuracy factor，AF）、R2对修正的Gompertz、修正的Logistic和Baranyi方程的拟合优度进行评价，修正的Logistic方程的RSS和RMSE均最小，BF和AF均最接近1，修正的Logistic模型的拟合优度最佳，适用性最强，水产品中腐败希瓦氏菌的生长情况能通过修正的Logistic模型得到较好地预测。

关键词: 腐败希瓦氏菌, 温度, 生长动力学, 拟合优度

Abstract:

The specific spoilage bacterium Shewanella putrefaciens isolated from refrigerated large yellow croaker was
grown at 5, 8, 15 and 25 ℃ in tryptone soy broth and the modified Gompertz equation, the modified Logistic equation and
the Baranyi equation were applied to fit its growth kinetic models. Further, a second-order model was established using
the Belehradek equation indicating the influence of temperature on the growth kinetics of Shewanella putrefaciens, and the
goodness of fit and applicability of the models were examined. The results showed that temperature remarkably affected the
growth kinetics of Shewanella putrefaciens. The growth of Shewanella putrefaciens was significantly inhibited at 5 ℃ with
a longer lag phase. At temperature up to 25 ℃, the lag phase of growth was reduced significantly. The specific growth rate
increased with temperature. Moreover, linear relationships between temperature and either the specific growth rate or the lag
period of growth were observed. Root mean square error (RMSE), residual sum of squares (RSS), bias factor (BF), accuracy
factor (AF) and R2 were used to evaluate the goodness of fit of the developed models. The results showed that the evaluation
parameters RSS, RMSE of the modified Logistic model were minimal, and BF and AF values were close to 1, suggesting that
the goodness of fit and applicability of the modified Logistic model were the best. Therefore, the modified Logistic model
can be used to predict the growth of Shewanella putrefaciens in aquatic products.

Key words: Shewanella putrefaciens, temperature, growth kinetics, goodness of fit

中图分类号:

S983

朱彦祺，郭全友，李保国，姜朝军. 不同温度下腐败希瓦氏菌（Shewanella putrefaciens）生长动力学模型的比较与评价[J]. 食品科学, doi: 10.7506/spkx1002-6630-201613026.

ZHU Yanqi, GUO Quanyou, LI Baoguo, JIANG Chaojun. Comparison and Evaluation of Models for the Growth of Shewanella putrefaciens at Different Temperatures[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613026.

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