莲子红衣多糖的分离纯化及结构表征

doi:10.7506/spkx1002-6630-201615016

食品科学

莲子红衣多糖的分离纯化及结构表征

高航1，高延芬2，徐虹1,*

1.北京工商大学北京市食品添加剂工程技术研究中心，北京食品营养与人类健康高精尖创新中心，北京 100048；2.深圳市农科集团有限公司，广东深圳 518040

出版日期:2016-08-15 发布日期:2016-08-30

Separation, Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds

GAO Hang1, GAO Yanfen2, XU Hong1,*

1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; 2. Shenzhen Nongke Group Co. Ltd.,Shenzhen 518040, China

Online:2016-08-15 Published:2016-08-30

摘要/Abstract

摘要：

以莲子红衣为实验对象，将其中的多糖进行分离、纯化并表征结构。采用酶提醇析法提取莲子红衣粗多糖，选择DEAE-C阴离子交换树脂和Sephadex G-25凝胶柱层析，从莲子红衣粗多糖中分离纯化得到中性多糖和酸性多糖两种组分。经凝胶渗透色谱测定，中性和酸性多糖的重均分子质量分别为3.78×104 D和4.94×104 D，纯度分别为91.16%和90.24%。中性多糖组分由葡萄糖、木糖、甘露糖、半乳糖4 种单糖组成；酸性多糖组分由葡萄糖、木糖、半乳糖、岩藻糖、阿拉伯糖5 种单糖组成。红外光谱证明两种多糖中均含有糖类特征吸收峰，且为α构型的吡喃型多糖。核磁共振氢谱检测又进一步证实了两种多糖均为α构型。

关键词: 莲子红衣, 多糖, 分离纯化, 结构表征

Abstract:

The crude polysaccharides isolated from the red skin of lotus seeds by enzymatic extraction and ethanol
precipitation were separated into neutral and acidic polysaccharides by DEAE-C column chromatography and subsequent
Sephadex G-25 gel column chromatography. Gel permeation chromatography (GPC) analysis showed that the molecular
weights of the neutral and acidic polysaccharides were 3.78 × 104 D and 4.94 × 104 D with a purity of 91.16% and 90.24%,
respectively. The neutral polysaccharide was composed of 4 monosaccharides including glucose and xylose, while the
acidic polysaccharide was mainly composed of 5 monosaccharides including glucose, xylose and galactose. Infrared spectra
preliminarily confirmed that both purified fractions were pyranoses with α configuration. This conclusion was further proved
by nuclear magnetic resonance.

Key words: red skin of lotus seeds, polysaccharide, separation and purification, structural characterization

中图分类号:

TS201.2

高航，高延芬，徐虹. 莲子红衣多糖的分离纯化及结构表征[J]. 食品科学, doi: 10.7506/spkx1002-6630-201615016.

GAO Hang, GAO Yanfen, XU Hong. Separation, Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201615016.

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莲子红衣多糖的分离纯化及结构表征

Separation, Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds

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