荷花粉多糖显著减轻氟尿嘧啶所致小鼠肠道黏膜屏障损伤

doi:10.7506/spkx1002-6630-201615035

食品科学

荷花粉多糖显著减轻氟尿嘧啶所致小鼠肠道黏膜屏障损伤

方小明1,2，田文礼2，张晓琳1，彭文君2，肖红伟1，高凌宇2，王灿红3，高振江1,*

1.中国农业大学工学院，北京 100083；2.中国农业科学院蜜蜂研究所，北京 100093；
3.中国医学科学院北京协和医学院药用植物研究所，北京 100193

出版日期:2016-08-15 发布日期:2016-08-30

Lotus Bee Pollen Polysaccharides Significantly Relieve Intestinal Mucosal Barrier Damage in Mice Caused by Fluorouracil

FANG Xiaoming1,2, TIAN Wenli2, ZHANG Xiaolin1, PENG Wenjun2, XIAO Hongwei1, GAO Lingyu2, WANG Canhong3, GAO Zhenjiang1,*

1. College of Engineering, China Agricultural University, Beijing 100083, China; 2. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China

Online:2016-08-15 Published:2016-08-30

摘要/Abstract

摘要：

目的：探讨不同干燥方法提取的荷花粉多糖（lotus bee pollen polysaccharides，LP）对5-氟尿嘧啶（5-fluorouracil，5-Fu）所致小鼠肠道黏膜损伤及功能失调的改善作用。方法：分别采用热风干燥与真空脉动干燥的方法对荷花粉进行干燥，并提取多糖。建立5-Fu小鼠肠黏膜炎模型，随机分组、给药。炭末推进法测定小鼠的肠推进率，并测量小鼠结肠长度；计算全肠道派氏集合淋巴结（Peyer’s patches，PPs）的面积；取小鼠结肠组织进行病理组织切片观察；生物化学方法检测PPs匀浆上清液中活性氧（reactive oxygen species，ROS）和谷胱甘肽（glutathione，GSH）含量、过氧化氢酶（catalase，CAT）和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力；酶联免疫吸附（enzyme-linked immunosorbent assay，ELISA）法检测PPs匀浆上清中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的含量。结果：与正常组相比，5-Fu可显著抑制小鼠肠道的推进功能（P＜0.05）。热风干燥与真空脉动干燥样品中提取的LP能减缓5-Fu所致的结肠缩短（P＜0.05），显著或极显著增加小鼠肠道PPs的面积（P＜0.05或P＜0.01）；此外，与5-Fu组比较，LP与5-Fu联合应用能明显促进小鼠肠道的推进功能（P＜0.05），升高GSH含量、CAT和GSH-Px活力（P＜0.01或P＜0.05），明显减少TNF-α含量（P＜0.05或P＜0.01）；结肠病理组织切片显示LP能明显减轻5-Fu所致的小鼠结肠黏膜上皮杯状细胞丢失、隐窝深度变浅及炎症细胞浸润。结论：LP能够减轻5-Fu所致小鼠结肠黏膜炎，改善5-Fu所致的肠道传输功能失调，保护5-Fu对肠道的氧化损伤，其中真空脉动干燥样品中提取的花粉多糖效果更好。

关键词: 荷花粉多糖, 5-氟尿嘧啶, 肠黏膜屏障, 氧化损伤, 干燥方式

Abstract:

Objective: To investigate the protective effects of polysaccharides (LP) extracted from lotus bee pollen dried
by different drying methods on intestinal mucosal inflammation and intestinal barrier dysfunctions in mice induced by
5-fluorouracil (5-Fu). Methods: Mouse models of intestinal mucosal inflammation were established by induction with 5-Fu
and the model mice were grouped randomly and subjected to drug administration. The carbon-propelling rate was tested,
and colon length and Peyer’s patches (PPs) area were measured. The colon tissue was stained with hematoxylin-eosin (HE)
for histological section observation. Reactive oxygen species (ROS) and glutathione (GSH) contents, and catalase (CAT) and
GSH-Px activities in the supernatant of PPs homogenate were evaluated by biochemical assays. Tumor necrosis factor-α (TNF-α)
in the supernatant of colon tissue homogenate was determined through ELISA. Results: Compared with the normal group,
intraperitoneal injection of 5-Fu to mice significantly inhibited small intestinal transit (P < 0.05). LP extracted from lotus bee pollen
dried by hot air or vacuum pulsed drying improved colon shortening caused by 5-Fu (P < 0.05) and resulted in a significant or highly
significant increase of PPs area (P < 0.05 or P < 0.01). Moreover, compared with the 5-Fu-induced model group, LP combined with
5-Fu could significantly promote intestinal transit (P < 0.05), increase GSH content, and CAT and GSH-Px activities (P < 0.01 or
P < 0.05), and decrease obviously TNF-α contents (P < 0.05 or P < 0.01). HE staining results showed that LP could significantly
reduce the loss of colonic mucosa epithelium goblet cells, inflammatory cell infiltration and more shallow crypt depth induced
by 5-Fu. Conclusion: LP could relieve intestinal mucosal damage, improve intestinal transit dysfunction and protect against
intestinal oxidative damage in mice induced by 5-Fu. The polysaccharides extracted from lotus bee pollen subjected to vacuum
pulsed drying exhibited better activity than the polysaccharides extracted from hot air dried lotus bee pollen.

Key words: lotus bee pollen polysaccharide, 5-fluorouracil, intestinal mucosal barrier, oxidative damage, drying method

中图分类号:

TS201

方小明，田文礼，张晓琳，彭文君，肖红伟，高凌宇，王灿红，高振江. 荷花粉多糖显著减轻氟尿嘧啶所致小鼠肠道黏膜屏障损伤[J]. 食品科学, doi: 10.7506/spkx1002-6630-201615035.

FANG Xiaoming, TIAN Wenli, ZHANG Xiaolin, PENG Wenjun, XIAO Hongwei, GAO Lingyu, WANG Canhong, GAO Zhenjiang. Lotus Bee Pollen Polysaccharides Significantly Relieve Intestinal Mucosal Barrier Damage in Mice Caused by Fluorouracil[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201615035.

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荷花粉多糖显著减轻氟尿嘧啶所致小鼠肠道黏膜屏障损伤

Lotus Bee Pollen Polysaccharides Significantly Relieve Intestinal Mucosal Barrier Damage in Mice Caused by Fluorouracil

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