关键词: MiSeq高通量测序, 臭氧-生物活性炭, 饮用水处理, 细菌多样性食品

Abstract:

Illumina MiSeq high-throughput sequencing technology was used to study the bacterial diversity and abundance
of the effluent water from each unit process in O3-BAC water treatment. A total of 196 809 16S rDNA sequences were
acquired, which were classified as 522 genera in 38 phyla. The results showed that there was high bacterial community
diversity in the processing unit effluent. Pre-ozonation and ozone oxidation treatment of water influenced most significantly
bacterial diversity and abundance. Ozone could kill some bacteria which were hard to be killed by chlorine disinfection in
a reasonable ozone concentration range. Flocculation and sand filtration could recover the bacterial diversity in water, as
demonstrated number of bacterial species. In biological activated carbon filter treatment, bacterial diversity was increased
and their abundance distribution was more uniform, which would propose higher requirements for later disinfection technics.

Key words: MiSeq high-throughput sequencing, ozone-biological activated carbon (O3-BAC), water treatment, bacterial diversity