Salmonella hadar对喹诺酮类药物耐药性及其耐药基因分析

doi:10.7506/spkx1002-6630-201617025

摘要/Abstract

摘要：

目的：研究新疆乌鲁木齐市部分农贸市场内检出的21 株Salmonella hadar对2 种喹诺酮类抗生素的药敏性及相关耐药基因，更好地了解耐药性的产生和传播途径，确保食品安全。方法：用琼脂稀释法测定Salmonella hadar的药敏性，用聚合酶链式反应和测定基因序列的方法确定耐药沙门氏菌中与喹诺酮类抗生素耐药性相关的喹诺酮类抗性决定区突变基因以及质粒携带的耐药基因。结果：21 株Salmonella hadar对萘啶酮酸的耐药率达100%，对环丙沙星表现为敏感；qnrB、qnrA、qnrS基因的检出率分别为52.30%、4.76%、4.76%；21 株Salmonella hadar均为gyrA和parC基因同时突变，gyrA基因的突变类型是Ser83Phe，parC基因的突变类型是Thr57Ser。结论：新疆乌鲁木齐Salmonella hadar对喹诺酮类药物的耐药状况应当予以关注，其耐药决定区突变基因及质粒携带的耐药基因在一定程度上会影响Salmonella hadar的耐药机制。

关键词: 沙门氏菌, 喹诺酮类药物, 耐药性, 聚合酶链式反应, 耐药基因

Abstract:

Objective: To characterize the antimicrobial resistance to 2 kinds of quinolones and the related genes in
21 Salmonella hadar isolates obtained from poultry and meat samples from some farmer’s markets in Ürümqi for the purpose
of getting a better understanding of the pathways of generation and transmission of antimicrobial resistance and consequently
ensuring food safety. Methods: The drug sensitivity of Salmonella hadar was evaluated by agar dilution method. In addition,
PCR and gene sequencing were used to detect the presence of mutations in the quinolone resistance-determining region
(QRDR) and plasmid-mediated quinolone resistance genes. Results: The resistance rates of these 21 Salmonella hadar
isolates were 100% to nalidixic acid and they were sensitive to ciprofloxacin. The plasmid-mediated quinolone resistance
genes including qnrB (52.3%), qnrA (4.76%), and qnrS (4.76%) were identified. The 21 Salmonella hadar isolates had
mutations in the gyrA and parC genes at the same time. Among 21 Salmonella hadar isolates, the common mutations
appeared at Ser83Phe and Thr57Ser in the gyrA and parC genes, respectively. Conclusion: There should be concerns about
the serious situation of antimicrobial resistance in Salmonella hadar isolates from poultry and meat in Ürümqi. In addition,
the quinolone resistance-determining region (QRDR) mutations and the plasmid-mediated quinolone resistance genes may
affect the antimicrobial resistance of Salmonella hadar.

Key words: Salmonella, quinolone drugs, drug resistance, polymerase chain reaction (PCR), drug resistance gene

中图分类号:

TS201.3

吴浩天，武运，尹明远，古丽娜孜，王威，张亚南，田歌，马文瑞. Salmonella hadar对喹诺酮类药物耐药性及其耐药基因分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201617025.

WU Haotian, WU Yun, YIN Mingyuan, GULINAZI, WANG Wei, ZHANG Yanan, TIAN Ge, MA Wenrui. Quinolone Resistance and Related Genes in Salmonella hadar[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201617025.

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Salmonella hadar对喹诺酮类药物耐药性及其耐药基因分析

Quinolone Resistance and Related Genes in Salmonella hadar

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