食品科学

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米曲霉Aspergillus oryzae As-W.6对脱氧雪腐镰刀菌烯醇的降解效果

吴 娱,王开萍,唐正江,汤 飞,刘国庆*   

  1. 合肥工业大学食品科学与工程学院,安徽 合肥 230009
  • 出版日期:2016-09-15 发布日期:2016-09-22

Degradation Efficiency of Deoxynivalenol by Aspergillus oryzae As-W.6

WU Yu, WANG Kaiping, TANG Zhengjiang, TANG Fei, LIU Guoqing*   

  1. College of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2016-09-15 Published:2016-09-22

摘要:

研究米曲霉Aspergillus oryzae As-W.6对脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)的降解作用。经高效液相色谱(high performance liquid chromatography,HPLC)检测,不同初始质量浓度的DON经菌株As-W.6发酵7 d后,降解率均在35%以上;发酵14 d后,降解率均在90%以上;同时该菌株中提取的降解酶对不同初始质量浓度DON的降解率均达70%以上。对降解产物采用高效液相色谱-质谱(HPLC-mass spectrometry,HPLC-MS)分析检测,结果显示DON经该降解菌降解后,相对分子质量从原来的295.1变为281.2,减少了13.9,说明DON确实被该菌降解成为另一种物质。

关键词: 脱氧雪腐镰刀菌烯醇, 米曲霉, 生物降解, 降解产物

Abstract:

This research work was conducted to investigate the degradation of deoxynivalenol (DON) by Aspergillus oryzae
As-W.6, which was isolated and preserved in our laboratory. Results showed the degradation rate of DON at all initial
concentrations investigated was greater than 35% after 7 d fermentation by strain As-W.6 and reached higher than 90%
with fermentation time up to 14 d. Moreover, the strain could degrade more than 70% of DON with the enzyme produced
by it. HPLC-MS analysis revealed that the relative molecular mass of DON was decreased from 295.1 to 281.2 after the
degradation, a decrease of 13.9, suggesting that DON was degraded into another substance by As-W.6.

Key words: deoxynivalenol, Aspergillus oryzae, biodegradation, degradation products

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