食品科学

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锦葵色素葡萄糖苷和锦葵色素半乳糖苷抑制内皮细胞氧化应激作用

朱运明1,2,黄午阳1,*,闵伟红2,隋中泉3,李春阳1   

  1. 1.江苏省农业科学院农产品加工研究所,江苏 南京 210014;2.吉林农业大学食品科学与工程学院,吉林 长春 130118;
    3.上海交通大学农业与生物学院,上海 200240
  • 出版日期:2016-09-15 发布日期:2016-09-22

Effects of Malvidin Glycosides on Ang II-Induced Oxidative Stress in Endothelial Cells

ZHU Yunming1,2, HUANG Wuyang1,*, MIN Weihong2, SUI Zhongquan3, LI Chunyang1   

  1. 1. Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;
    3. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2016-09-15 Published:2016-09-22

摘要:

目的:研究血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)对人脐静脉内皮细胞(human umbilical vein endothelialcells,HUVEC)中活性氧(reactive oxygen species,ROS)、黄嘌呤氧化酶-1(xanthine oxidase-1,XO-1)、血红素氧化酶-1(heme oxygenase-1,HO-1)含量和超氧化物歧化酶(superoxide dismutase,SOD)活性的影响,确定最佳AngⅡ诱导浓度,探讨不同浓度锦葵色素葡萄糖苷(malvidin-3-glucoside,Mv-3-glc)和锦葵色素半乳糖苷(malvidin-3-galactoside,Mv-3-gal)对AngⅡ诱导的血管内皮细胞氧化应激的抑制作用机理。方法:通过体外培养HUVEC,采用酶联免疫吸附分析法分别测定HUVEC中SOD活力、XO-1和HO-1含量,并采用荧光探针方法检测细胞中ROS水平变化。结果:在HUVEC中,AngⅡ可诱导XO-1的表达,抑制HO-1的表达,AngⅡ在浓度10-2 μmol/L时对HUVEC氧化应激诱导最为显著。不同浓度的Mv-3-glc、Mv-3-gal及其混合物均可显著抑制AngⅡ诱导的内皮细胞氧化应激,通过抑制XO-1蛋白过表达,增加HO-1和SOD表达量,改善细胞抗氧化防御系统,从而减少细胞内ROS水平,达到氧化保护作用,且Mv-3-glc比Mv-3-gal抗氧化活性更强。结论:锦葵色素葡萄糖苷和锦葵色素半乳糖苷具有抑制内皮细胞氧化应激的作用。

关键词: 锦葵色素葡萄糖苷, 锦葵色素半乳糖苷, 内皮细胞, 血管紧张素Ⅱ, 活性氧, 氧化应激

Abstract:

The objective of this research was to investigate the antioxidant role in endothelial cells. The influence of
angiotensin II (Ang II) on the expression of xanthine oxidase-1 (XO-1) and heme oxygenase-1 (HO-1) in human umbilical
vein endothelial cells (HUVECs), and the changes in reactive oxygen species (ROS), superoxide dismutase (SOD), XO-1,
and HO-1 caused by two malvidin glycosides malvidin-3-glucoside (Mv-3-glc) and malvidin-3-galactoside (Mv-3-gal) were
investigated. The levels of XO-1, HO-1, and SOD were evaluated by enzyme linked inmunosorbent assay (ELISA) kits.
ROS level was detected under a fluorescence microplate. Compared with the control group, the expression of XO-1 protein
was significantly increased and the expression of HO-1 protein was significantly decreased in the 10-2 μmol/L Ang II-treated
group. Different concentrations of Mv-3-glc and Mv-3-gal as well as their mixture greatly decreased the levels of ROS
and XO-1, but increased the levels of SOD and HO-1. Glycosides improved the antioxidant capacity of malvidin to a great
extent. The antioxidant activity of Mv-3-glc was more pronounced than that of Mv-3-gal. Variation in malvidin glycosides
had a significant impact on Ang II-induced oxidative stress to different extents. Therefore, malvidin glycosides can protect
cells from oxidative deterioration and thus used to prevent diseases associated with oxidative stress.

Key words: malvidin-3-glucoside, malvidin-3-galactoside, endothelial cells, angiotensin II (Ang II), reactive oxygenspecies (ROS), oxidative stress

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