食品科学

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核酸适配体技术在食品中霉菌毒素检测的研究进展

杜兵耀1,2,3,4,文 芳1,3,4,*,王加启1,3,4,郑 楠1,3,4,张养东1,3,4,赵圣国1,3,4   

  1. 1.中国农业科学院北京畜牧兽医研究所,农业部奶产品质量安全风险评估实验室(北京),北京 100193;
    2.新疆农业大学动物科学学院,乌鲁木齐 830052;3.农业部奶及奶制品质量监督检验测试中心(北京),北京 100193;
    4.中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193
  • 出版日期:2016-09-15 发布日期:2016-09-22

Advances in the Application of Aptamers to Detect Mycotoxin in Foods

DU Bingyao1,2,3,4, WEN Fang1,3,4,*, WANG Jiaqi1,3,4, ZHENG Nan1,3,4, ZHANG Yangdong1,3,4, ZHAO Shengguo1,3,4   

  1. 1. Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Animal Science, Xinjiang Agricultural University, Ürümqi 830052, China; 3. Milk and Dairy Product Inspection Center (Beijing), Ministry of Agriculture, Beijing 100193, China; 4. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Online:2016-09-15 Published:2016-09-22

摘要:

近年来,我国食品质量安全事件频发,对人们的身体健康构成严重威胁,人们对食品质量安全也逐渐重视起来。核酸适配体是利用指数富集的配基系统进化技术体外筛选得到的单链DNA或RNA片段,能特异性地结合目标分子。具有操作简单、灵敏度高、选择性好、检测成本低等优点,已经广泛地应用于食品中蛋白质、霉菌毒素等方面的检测。本文主要综述了核酸适配体技术检测食品中霉菌毒素的研究进展。

关键词: 核酸适配体, 检测, 食品, 霉菌毒素

Abstract:

In recent years, food quality and safety events have occurred frequently in China and they have posed serious
health hazards. Consequently, there are rising concerns about the issue of food safety and quality. Aptamers are singlestranded
DNA or RNA molecules obtained by systematic evolution of ligands by exponential enrichment (SELEX). They
possess high affinity to target molecules. Aptamers have the advantages of low cost, simple operation, high sensitivity and
selectivity. Therefore, they have been applied for the determination of proteins and mycotoxins. This paper reviews the latest
advances in the application of aptamer-based methods for the detection of mycotoxins in foods.

Key words: nucleic acid aptamer, detection, food, mycotoxin

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