食品科学 ›› 2016, Vol. 37 ›› Issue (20): 24-30.doi: 10.7506/spkx1002-6630-201620005

• 工艺技术 • 上一篇    下一篇

响应面试验优化辣木叶多酚超声辅助提取工艺及其抗氧化活性

裴 斐,陶虹伶,蔡丽娟,魏琛琛,袁一鸣,杨文建,胡秋辉   

  1. 南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,
    江苏高校粮油质量安全控制及深加工重点实验室,江苏 南京 210023 ?
  • 收稿日期:2016-10-27 修回日期:2016-10-27 出版日期:2016-10-25 发布日期:2016-12-01
  • 通讯作者: 胡秋辉-
  • 基金资助:

    江苏高校优势学科建设工程资助项目(PAPD)

Optimization of Polyphenols from Moringa oleifera Lam. Leaves by Ultrasound-assisted Extraction Using Response Surface Methodology and Their Antioxidant Activities

PEI Fei, TAO Hongling, CAI Lijuan, WEI Chenchen, YUAN Yiming, YANG Wenjian, HU Qiuhui   

  1. Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing,
    College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China
  • Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01
  • Contact: HU Qiuhui

摘要:

为研究辣木叶多酚超声辅助提取工艺,明确辣木叶多酚体外抗氧化活性,选取超声功率、超声时间、超声温度和料液比为考察指标,研究不同工艺参数对辣木叶多酚提取量的影响,并采用响应面法优化辣木叶多酚提取工艺。此外,研究辣木叶多酚还原力及其对DPPH自由基、超氧阴离子自由基清除能力。结果表明,超声辅助提取辣木叶多酚最优工艺为:超声时间19.5 min、料液比1∶30(g/mL)、超声温度20.2 ℃、超声功率250 W。在此条件下,辣木叶多酚提取量为(25.14±0.46) mg/g。辣木叶多酚具有较强的体外抗氧化活性,其还原力、DPPH自由基和超氧阴离子自由基清除能力分别达到同等质量浓度VC的81.25%、94.15%和75.05%。该研究为辣木叶多酚等生物活性成分高效制备与抗氧化剂的深度开发提供理论依据。

关键词: 辣木叶, 多酚, 超声辅助提取, 响应面, 抗氧化

Abstract:

In order to obtain the best ultrasound-assistedextracting conditions for polyphenols from Moringa oleifera
Lam. leaves, and clarified their antioxidant activities. Response surface methodology was used to explore the effects of
ultrasonic power, extraction time, extraction temperature, and solid to liquid ratio on extraction rate. Moreover, the reducing
power, the DPPH radical scavenging activities and the O2-· scavenging effect of polyphenols from Moringa oleifera Lam.
leaves were also investigated. The results showed that the best extraction condition were as follows: the extraction time of
19.5 min, the solid to liquid ratio of 1:30 (g/mL), the extraction temperature of 20.2 ℃, and the ultrasonic power of 250 W.
Under the optimal extractions, the extraction rate of phycoerythrin was (25.14 ± 0.46) mg/g. In addition, the polyphenols
from Moringa oleifera Lam. leaves. have strong anti-oxidative, and the reducing power, the DPPH radical scavenging activities
and the O2-· scavenging effect were 81.25%, 94.15% and 75.05% of L-ascorbic acid, respectively. This research could provide the
theoretical basis for higher preparation and antioxidants development of Moringa oleifera Lam. leaves.

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