食品科学

• 生物工程 • 上一篇    下一篇

抑制口腔变异链球菌的乳酸菌筛选及其抑菌机理

郭夏蕾,张 健,杨贞耐*   

  1. 北京工商大学 北京食品营养与人类健康高精尖创新中心,食品质量与安全北京实验室,北京 100048
  • 出版日期:2016-10-15 发布日期:2016-12-01

Screening of Lactic Acid Bacteria for Inhibiting Oral Streptococcus mutans and Preliminary Study of Antibacterial Mechanism

GUO Xialei, ZHANG Jian, YANG Zhennai*   

  1. Beijing Laboratory of Food Quality and Safety, Beijing Advanced Innovation Center for Food Nutrition and Human Health,
    Beijing Technology and Business University, Beijing 100048, China
  • Online:2016-10-15 Published:2016-12-01

摘要:

利用乳酸菌能抑制有害菌的特性,从实验室保藏的乳酸菌中筛选能抑制口腔变异链球菌的菌株,并测定了筛选菌株的最小抑菌浓度(minimal inhibitory concentration,MIC),以及其对变异链球菌菌膜形成和黏附性的影响,初步研究其抑菌机理。结果表明,有5 株植物乳杆菌(Lactobacillus plantarum) K25、SKT109、YW3-2、YW5-1、YW1-1对变异链球菌具有明显的抑制作用。4 株变异链球菌指示菌中,变异链球菌(Streptococcus mutans)1.2499、1.2500对植物乳杆菌比较敏感。植物乳杆菌K25对变异链球菌的抑制作用比其他4株植物乳杆菌的作用更明显,其MIC最低(1.25×107~2.50×107 CFU/mL)。同时,菌株K25对变异链球菌菌膜形成的抑制率较高,还显著地降低变异链球菌的黏附率。对菌株K25抑菌机理的初步研究表明,其代谢过程中产生的乳酸和过氧化氢起到了主要的抑菌作用。

关键词: 变异链球菌, 益生菌, 植物乳杆菌, 抑菌活性

Abstract:

Considering that lactic acid bacteria (LAB) that can inhibit harmful bacteria, LAB strains stored in our laboratory
were screened for inhibiting oral Streptococcus mutans. The screened strains were measured for minimum inhibitory
concentration (MIC) and inhibitory effect on biofilm formation adhesion properties of S. mutans, and the underlying
mechanism was preliminarily studied as well. The results showed that 5 Lactobacillus plantarum strains including K25,
SKT109, YW3-2, YW5-1 and YW1-1 exhibited clear inhibition against S. mutans. Among 4 indicator S. mutans strains,
1.2499 and 1.2500 were more sensitive to L. plantarum. L. plantarum K25 showed stronger inhibition than 4 other strains
against S. mutans. The MIC value (1.25 × 107–2.50 × 107 CFU/mL) of strain K25 was the lowest, the inhibition rate on
biofilm formation of S. mutans was significantly higher, and the inhibitory effect on the adhesion ability of S. mutans was
clear. Preliminary studies on the antibacterial mechanism of L. plantarum K25 indicated that lactic acid and hydrogen
peroxide produced by the strain played major roles in this activity.

Key words: Streptococcus mutans, probiotic, Lactobacillus plantarum, inhibitory activity

中图分类号: