荞麦壳黄酮提取物对2型糖尿病大鼠的血糖改善作用及其机制

摘要/Abstract

摘要： 目的：研究荞麦壳黄酮提取物（BHEs）对2型糖尿病大鼠血糖水平改善作用和可能的机制。方法：体外试验中，测定了BHEs抑制α-葡萄糖苷酶和α-淀粉酶活性，并采用Lineweaver-Burk (L-B)作图法确定其抑制反应的类型。体内试验中，高脂饮食与注射链脲佐菌素(STZ)复合诱导制备2型糖尿病大鼠模型，分为正常组（未诱导，蒸馏水，标记为Normal），模型组（诱导，蒸馏水，标记为Diabetes），阳性对照组（盐酸二甲双胍，100mg/kg/d，标记为Met）和BHEs低、中、高剂量组（50、100、200 mg/kg/d，分别标记为LBHEs、MBHEs、HBHEs）。灌胃给药，每天一次，连续干预28d，观察大鼠体重、空腹血糖（FBG）、空腹胰岛素（FINS）和C肽，并进行口服糖耐量试验（OGTT）。结果：BHEs在α-葡萄糖苷酶-麦芽糖体系中抑制活性成剂量依赖关系，当BHEs 浓度为1mg/mL时抑制率为41.12%，酶促动力学数据表明其为竞争型抑制。STZ诱导的2型糖尿病大鼠试验结果显示，与正常组相比，模型组大鼠体重减少了33.1%， FBG升高到17.85±2.25mmol/L，而FINS，C肽水平分别降低了24.80%和12.77%（P＜0.05）；与模型组相比，BHEs试验组改善糖尿病大鼠体重减少，且干预28天后MBHEs组和HBHEs组大鼠FBG与干预前比较分别降低了39.4%和48.2%（P＜0.05），OGTT曲线下面积（AUC）降低了47.36%、59.47%（P＜0.05），MBHEs组和HBHE组同时改善大鼠血清FINS和C肽水平，与模型组相比分别提高了32.79%、34.44%和7.4%、13.08%（P＜0.05）。结论：荞麦壳黄酮提取物抑制α-葡萄糖苷酶活性和修复胰岛细胞功能的双重机制改善STZ诱导的2型糖尿病大鼠的血糖指标，并呈现一定的剂量关系。

关键词: 荞麦壳黄酮提取物, α-葡萄糖苷酶, 2型糖尿病, 血糖, 胰岛素, C肽

Abstract: Purpose: To evaluate the improvement effect on blood glucose levels in type 2 diabetic rats using the extract from buckwheat shell (BHEs) and its possible mechanisms. Methods: BHEs inhibition of α-glucosidase and α-amylase activities were measured In vitro, and the type of response was definite by Line weaver-Burk (L-B) wave method. In vivo，the experimental group was given high fat diet and Streptozotocin(STZ) intraperitoneally to induce type 2 diabetic model，and the rats were randomly divided into control（non-induction, water, Normal group） model group（induction, water, Diabetes group），positive control group（Metformin hydrochloride，100mg/kg/d, Met group），low-dose of BHEs group（50 mg/kg/d，LBHEs），medium-dose of BHEs group （100 mg/kg/d，MBHEs）and high-dose of BHEs group（200 mg/kg/d，HBHEs），respectively. They were given relevant gavage administration once a day for 28 days. The body weight，fasting blood glucose（FBG），fasting insulin（FINS），fasting C-peptide and Oral glucose tolerance test（OGTT）were determined. Results: BHEs indicated the inhibitory activity in α-glucosidase-maltose system with a dose-response relationship; the inhibition ratio is 41.12% while the concentration of BHEs is 1mg/mL, the date of enzyme kinetics showed it is the competitive mode of inhibition. In model group , the weight has loosed 33.1%, FBG has raised to 17.85±2.25mmol/L as well as the level of FINS and C peptide reduced 24.80%, 12.77%（P＜0.05）, respectively, which compared with normal group. Compared with the model group, the BHEs group weight loosed significantly and after 28 days intervened, the FBG of MBHEs group and HBHEs group decreased 39.4% and 48.2%, respectively. The area under the curve (AUC) declined 47.36% and 59.47%，respectively（P＜0.05）.Compared with model group, both the MBHEs and the HBHEs group improved the levels of FINS and C-peptide to 32.79%, 34.44% and 7.4%，13.08%，respectively（P＜0.05）. Conclusions: BHEs can improve blood glucose level in a dose-dependent manner in type 2 diabetes by STZ induced as α-glucosidase inhibitors and repair of islet cell function.

Key words: Buckwheat Hull Extracts, α-glucosidase, diabetes mellitus type 2, Blood glucose, insulin, C-peptide

中图分类号:

TS210.9 TS218

朴春红李鹏程张羽赵梓瀛王玉华刘俊梅于寒松张岚李想. 荞麦壳黄酮提取物对2型糖尿病大鼠的血糖改善作用及其机制[J]. 食品科学, 0, (): 0-0.

参考文献

[1] Guariguata L, Whiting D R, Hambleton I, et al. Global estimates of diabetes prevalence for 2013 and projections for 2035[J]. Diabetes research and clinical practice, 2014, 103(2): 137-149.
[2] Sarah W, Gojka R, Anders G, et al. Global prevalence of diabetes[J]. Diabetes care, 2004, 27(5): 1047-1053.
[3] Action to Control Cardiovascular Risk in Diabetes Study Group. Effects of intensive glucose lowering in type 2 diabetes[J]. N Engl j Med, 2008, 2008(358): 2545-2559.
[4] International Diabetes Federation Guideline Development Group. Global guideline for type 2 diabetes[J]. Diabetes research and clinical practice, 2014, 104(1): 1.
[5] FORCE T. AACE comprehensive diabetes management algorithm[J]. Endocrine Practice, 2013, 19(2): 327.
[6] 秦培友. 我国主要荞麦品种资源品质评价及加工处理对荞麦成分和活性的影响[D]. 北京: 中国农业科学院, 2012.
[7] 阎红. 荞麦的应用研究及展望[J]. 食品工业科技, 2011 (1): 363-365.
[8] 余红, 王志路, 韩淑英, 等. 荞麦种子提取物对 α-糖苷酶及餐后血糖影响的实验研究[J]. 四川中医, 2010, 28(5): 60-62.
[9] 张敏. 荞麦凉茶工艺研究及其对 Ⅱ 型糖尿病小鼠降血糖作用[D]. 西北农林科技大学, 2014.
[10] 俞浩, 毛斌斌, 周国梁, 等. 白背三七总黄酮对糖尿病大鼠的降血糖作用[J]. 食品科学, 2013, 34(15): 295-298.
[11] 李超, 崔珏, 周小双, 等. 鼠曲草总黄酮改善糖尿病小鼠抗氧化功能的研究[J]. 食品科学, 2013, 34(21): 311-314.
[12] 李丹, 彭成, 谢晓芳. 黄酮类化合物治疗糖尿病及其并发症的研究进展[J]. 中国实验方剂学杂志, 2014, 20(11): 239-242.
[13] 朴春红, 刘丽苹, 初琦, 等. 热水法提取荞麦壳黄酮工艺优化及抗氧化活性[J]. 吉林农业大学学报, 2014, 36(6): 719-722,734.
[14] 陈旭清. 荞麦壳精深加工综合利用研究[D]. 陕西科技大学, 2014.
[15] XIAO J, HUO J, JIANG H, et al. Chemical compositions and bioactivities of crude polysaccharides from tea leaves beyond their useful date [J]. International journal of biological macromolecules, 2011, 49(5): 1143-51.
[16] 范文娅, 吴正钧, 季红, 等. 不同干酪乳杆菌菌株对 α-葡萄糖苷酶抑制作用的比较[J]. 食品与发酵工业, 2012, 38(4): 29-33.
[17] YANG X W, HUANG M Z, JIN Y S, et al. Phenolics from Bidens bipinnata and their amylase inhibitory properties [J]. Fitoterapia, 2012, 83(7): 1169-75.
[18] 林心君, 王麒又, 辛金钟, 等. 高成模率和高稳定性的糖尿病大鼠模型制备——高脂高糖膳食+ STZ 体重联合体表面积法构建糖尿病大鼠模型[J]. 中国老年学杂志, 2013, 33(09): 2051-2054.
[19] 邵俊伟, 蔡逊. 高脂饮食联合链脲佐菌素建立 2 型糖尿病大鼠模型的研究进展[J]. 中国实验动物学报, 2014, 22(4): 90-93.
[20] 李晨岚. 以α-葡萄糖苷酶为靶向降糖物质的研究[D]. 武汉: 华中农业大学, 2008.
[21] 李艳琴, 周凤超, 陕方, 等. 荞麦麸皮提取物对 α-葡萄糖苷酶活性的影响[J]. 食品科学, 2010 (17): 10-13.
[22] 朱丽艳, 郭兰, 王瑞雪, 等. 荞麦花总黄酮和槲皮素对 α-葡萄糖苷酶活性的影响[J]. 时珍国医国药, 2010, 21(5): 1135-1136.
[23] 王斯慧, 白银花, 黄琬凌, 等. 苦荞黄酮对 α-淀粉酶的抑制作用研究[J]. 食品工业, 2012, 3: 109-111.
[24] 黄云霞, 张民, 张倩, 等. α-葡萄糖苷酶抑制剂筛选及其抑制类型研究[J]. 中国食品添加剂, 2014 (3): 96-99.
[25] 文丽霞, 吴正钧, 郭本恒. 两种 α-糖苷酶抑制剂体外筛选模型的改良与比较[J]. 天然产物研究与开发, 2015, 27(4): 681-686.
[26] 韩淑英, 张博男, 张静怡, 等. 荞麦花叶黄酮对 2型糖尿病大鼠血糖稳态的影响[J]. 第三军医大学学报, 2013, 35(23): 2597-2599.
[27] Banerjee M, Vats P. Reactive metabolites and antioxidant gene polymorphisms in type 2 diabetes mellitus[J]. Redox biology, 2014, 2: 170-177
[28] 周玉荣, 肖健青. 2 型糖尿病患者血清胰岛素及 C 肽水平变化的临床研究[J]. 检验医学与临床, 2013, 10(18): 2413-2415.
[29] IRUDAYARAJ S S, STALIN A, SUNIL C, et al. Antioxidant, antilipidemic and antidiabetic effects of ficusin with their effects on GLUT4 translocation and PPARgamma expression in type 2 diabetic rats [J]. Chemico-biological interactions, 2016,
[30] Salmanoglu D S, Gurpinar T, Vural K, et al. Melatonin and L-carnitin improves endothelial disfunction and oxidative stress in Type 2 diabetic rats[J]. Redox biology, 2016, 8: 199-204.
[31] Dogan A, Celik I, Kaya M S. Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats[J]. Journal of ethnopharmacology, 2015, 176: 243-251.