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黄酮醇糖苷与茶树品种适制性关系研究

戴伟东1,解东超1,吕美玲2,谭俊峰1,李朋亮1,吕海鹏1,林智1   

  1. 1. 中国农业科学院茶叶研究所
    2. 安捷伦科技(中国)有限公司
  • 收稿日期:2016-06-16 修回日期:2017-05-22 出版日期:2017-08-25 发布日期:2017-08-18
  • 通讯作者: 林智 E-mail:linz@mail.tricaas.com
  • 基金资助:
    国家自然科学基金地区科学基金;中国农业科学院创新工程;现代农业产业技术体系建设专项资金

Study on the Relation of Flavonol Glycoside and Processing Suitability of Tea Variety

  • Received:2016-06-16 Revised:2017-05-22 Online:2017-08-25 Published:2017-08-18

摘要: 对不同加工适制性的18 个茶树品种春季茶叶中10 种黄酮醇糖苷进行了超高效液相色谱-四极杆-飞行时间质谱联用检测,并计算了葡萄糖苷化黄酮醇与半乳糖苷化黄酮醇的比值。结果发现相比于适制绿茶的茶树品种,适制红茶品种茶叶中黄酮醇葡萄糖苷化水平较高,而半乳糖苷化水平较低。山柰酚-3-葡萄糖苷含量、山柰酚-3-葡萄糖酰芸香糖苷含量、山柰酚-3-葡萄糖苷/山柰酚-3-半乳糖苷比值、槲皮素-3-葡萄糖苷/槲皮素-3-半乳糖苷比值、杨梅素-3-葡萄糖苷/杨梅素-3-半乳糖苷比值在不同适制性品种间具有显著性差异(P<0.05)。其中山柰酚-3-葡萄糖苷含量和山柰酚-3-葡萄糖苷/山柰酚-3-半乳糖苷比值差异极显著(P<0.001),对于绿茶适制性品种和红茶适制性品种的判断准确率分别为100%和83%,具有作为指导茶树品种适制性生化指标的可能。

关键词: 茶叶, 品种适制性, 黄酮醇糖苷, 葡萄糖苷, 半乳糖苷, 液质联用

Abstract: The contents of 10 flavonol glycosides were determinated in 18 tea varieties using ultra high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-QTOF/MS). The ratios of flavonol glucoside to flavonol galactoside were also calculated. The results showed that the glucosylation level of flavonol is more vigorous in tea varieties of black tea processing suitability when compared with tea varieties of green tea processing suitability. Kaempferol-3-glucoside, kaempferol-3-glucosylrutinoside, kaempferol-3-glucoside to kaempferol-3-galactoside ratio, quercetin-3-glucoside to quercetin-3-galactoside ratio, myricetin-3-glucoside to myricetin-3-galactoside ratio showed significant difference (P < 0.05) among tea varieties of different processing suitability. The significant difference (P < 0.001) of kaempferol-3-glucoside and kaempferol-3-glucoside/kaempferol-3-galactoside ratio indicates the probability for the discrimination of processing suitability of tea variety. The accuracy of the discrimination for tea varieties of green and black tea processing suitability were 100% and 83%, respectively.

Key words: tea, processing suitability, flavonol glycoside, glucoside, galactoside, LC-MS

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