食品科学 ›› 2016, Vol. 37 ›› Issue (24): 313-323.doi: 10.7506/spkx1002-6630-201624049

• 包装贮运 • 上一篇    

6-苄氨基嘌呤减缓鲜切西兰花的衰老机理分析

刘红艳,胡花丽,罗淑芬,张雷刚,肖丽霞,李鹏霞   

  1. 1.扬州大学旅游烹饪学院,江苏 扬州 225127;2.江苏省农业科学院农产品加工研究所,江苏 南京 210014
  • 出版日期:2016-12-25 发布日期:2016-12-21
  • 基金资助:
    江苏省农业科技自主创新基金项目(CX(15)1048)

6-Benzylaminopurine Alleviates the Senescence of Fresh-Cut Broccoli through Inhibiting the Production of Ethylene

LIU Hongyan, HU Huali, LUO Shufen, ZHANG Leigang, XIAO Lixia, LI Pengxia   

  1. 1. School of Tourism and Culinary Science, Yangzhou University, Yangzhou 225127, China; 2. Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2016-12-25 Published:2016-12-21

摘要: 为研究6-苄氨基嘌呤(6-benzylaminopurine,6-BA)调控采后西兰花衰老的可能机理,以鲜切西兰花为材料,考察了30 mg/L 6-BA处理对鲜切西兰花内源6-BA含量、总叶绿素含量、菌落总数、乙烯释放量、衰老相关基因表达、乙烯合成相关酶活性及其基因表达的影响。结果表明:6-BA处理可有效延缓西兰花总叶绿素的降解,抑制其菌落总数上升;随着贮藏时间的延长,与对照西兰花内源6-BA含量不断下降不同,外源6-BA处理则维持了贮藏后期组织内较高的6-BA含量,但经煮熟后,处理和对照组的内源6-BA含量均大幅度下降;6-BA处理下调了叶绿素酶2、半胱氨酸蛋白酶5和富含脯氨酸蛋白基因的表达,延缓了叶绿素a/b结合蛋白1基因表达的下降;6-BA处理降低了乙烯合成酶活性及其基因的表达,显著抑制了西兰花的乙烯释放量。总之,外源6-BA维持了鲜切西兰花贮藏后期组织内较高的内源6-BA水平,从而抑制了乙烯的生成,最终减缓了西兰花的衰老进程。

关键词: 西兰花, 6-苄氨基嘌呤, 衰老, 乙烯

Abstract: To elucidate the possible mechanism by which 6-benzylaminopurine (6-BA) regulates the senescence of broccoli, the effects of 30 mg/L 6-BA on the endogenous 6-BA content, total chlorophyll content, total bacterial account, ethylene production, the expression of senescence-related genes, the activities and gene expression of the ethylene-forming enzymes in broccoli were investigated. The results showed that 6-BA treatment could significantly alleviate the degradation of total chlorophyll and inhibit the increase in total bacterial count in broccoli. The endogenous 6-BA content of control broccoli continuously decreased with the extent of storage time, while exogenous 6-BA treatment maintained higher endogenous 6-BA content at the later stage of storage. The endogenous 6-BA content in both the control and 6-BA treatment groups decreased after cooking. Additionally, 6-BA treatment down-regulated the expressions of chlorophyllase gene (2BoCLH2), cysteine protease gene (5BoCP5), proline rich protein gene (BoLSC810), and retarded the decline in the expression of chlorophyll a/b binding protein 1 (BoCAB1). Moreover, 6-BA treatment significantly suppressed ethylene production in broccoli, which might be associated with decreased activity of ethylene synthase and down-regulated expression of BoACS1 and BoACS2. These results suggested that exogenous 6-BA maintained higher endogenous 6-BA level at the later period of storage, thereby inhibited ethylene production, and finally slowed down the senescence of broccoli.

Key words: broccoli, 6-benzylaminopurine, senescence, ethylene

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