食品科学

• 基础研究 • 上一篇    下一篇

热处理大豆蛋白体外消化产物结构特征分析

王中江,张潇元,隋晓楠,齐宝坤,江连洲,李欣芮,姜 楠,黄天赐,张 鑫,李 杨*   

  1. 东北农业大学食品学院,黑龙江 哈尔滨 150030
  • 出版日期:2017-01-15 发布日期:2017-01-16

Structural Characteristics of in Vitro Digestion Products of Heat-Treated Soybean Protein

WANG Zhongjiang, ZHANG Xiaoyuan, SUI Xiaonan, QI Baokun, JIANG Lianzhou, LI Xinrui, JIANG Nan, HUANG Tianci, ZHANG Xin, LI Yang*   

  1. College of Food Science, Northeast Agricultural Univeristy, Harbin 150030, China
  • Online:2017-01-15 Published:2017-01-16

摘要: 以大豆分离蛋白为基础原料,运用多种蛋白分析检测手段,深入探讨70、80、85、90、100 ℃热处理条件对大豆分离蛋白体外模拟消化特性影响。从蛋白质水解度(the degree of hydrolysis,DH)曲线来看,随着热处理温度的升高,DH曲线呈现先上升后下降的变化趋势,而长时间也会使DH呈现下降的趋势。傅里叶转换红外光谱及拉曼光谱图分析显示,经不同温度热预处理样品的消化产物二级结构中α-螺旋结构含量较未经预处理样品的高,而β-折叠结构含量降低,β-转角与无规卷曲结构含量变化不明显;随着时间的延长,α-螺旋结构含量呈先升高后降低的变化趋势,而β-折叠结构含量先降低后升高。拉曼光谱中酪氨酸峰变化较小且不规律,热处理使色氨酸残基更趋近于“包埋态”。

关键词: 大豆蛋白, 体外模拟消化, 胃蛋白酶, 热处理, 结构特性

Abstract: The effect of thermal pretreatments at different temperatures (70, 80, 85, 90, and 100 ℃) on the in vitro
digestibility of soy protein isolate (SPI) was investigated using various protein analytical techniques. The curves of degree
of hydrolysis (DH) showed an upward and then downward trend with pretreatment temperature or time. FTIR and Raman
spectral analysis showed that thermal pretreatments resulted in a higher α-helix content and a lower β-sheet content in
digestion products of SPI compared with the un-pretreated sample, while no significant changes in β-turn or random coil
were observed. The α-helix content rose firstly and then fell, whereas the opposite trend was noted for β-sheet content. The
Raman spectral peaks of tyrosine revealed small and irregular changes, leading to a conclusion that heat-treated tryptophan
residues were more close to “embedding state”.

Key words: soy protein isolate (SPI), in vitro digestion, pepsin, heat treatment, structural characteristics

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