食品科学 ›› 2017, Vol. 38 ›› Issue (2): 15-19.doi: 10.7506/spkx1002-6630-201702003

• 生物工程 • 上一篇    下一篇

传统发酵锦州小菜中主要微生物多样性分析

孙慧君,张 颖,王洪玉,杨 梅,岳喜庆,乌日娜   

  1. 1.沈阳农业大学食品学院,辽宁 沈阳 110866;2.辽宁省农业经济学校现代农业技术系,辽宁 锦州 121001;3.沈阳农业大学园艺学院,辽宁 沈阳 110866;4.江南大学 食品科学与技术国家重点实验室,食品学院,江苏 无锡 214122
  • 出版日期:2017-01-25 发布日期:2017-01-16
  • 基金资助:
    国家自然科学基金面上项目(31471713);中国博士后科学基金项目(2014M560395);辽宁省农业领域青年科技创新人才培养计划项目(2014048);辽宁省高等学校优秀人才支持计划项目(LR2015059);江苏省博士后科研资助计划项目(1402071C)

Microbial Diversity of Traditional Fermented Jinzhou Pickles

SUN Huijun, ZHANG Ying, WANG Hongyu, YANG Mei, YUE Xiqing, WU Rina   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 2. College of Morden Agricultural Technology, Liaoning Agricultural Economic School, Jinzhou 121001, China; 3. College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China; 4. State Key Laboratory of Food Science and Technology, College of Food Science, Jiangnan University, Wuxi 214122, China
  • Online:2017-01-25 Published:2017-01-16

摘要: 通过探究锦州小菜的微生物群落组成,以期为改善其品质提供依据。利用聚合酶链式反应-变性梯度凝胶电泳对采集自10 份锦州地区传统发酵小菜中的微生物多样性进行研究,结果表明:共鉴定出5 种细菌,分别为植物乳杆菌(Lactobacillus plantarum)、食窦魏斯氏菌(Weissella cibaria)、Lactobacillus fabifermentans、希腊魏斯菌单菌(Weissella hellenica)、假单细胞菌(Psychromonas arctica);真菌菌属有3 种,分别为热带假丝酵母(Candiada tropicalis)、弯曲黏膜菌(Campylobacter mucosalis)、白色念珠菌(Candida albicans)。其中,主要细菌为植物乳杆菌、食窦魏斯氏菌,主要真菌为热带假丝酵母。

关键词: 锦州小菜, 聚合酶链式反应-变性梯度凝胶电泳, 多样性

Abstract: In order to improving the quality of Jinzhou pickles, this experiment studied the microbial community compositionof Jinzhou pickles. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was adopted to analyzethe microbial diversity of ten traditional pickle samples in Jinzhou area, Liaoning province. The results showed that fivebacterial strains were identified namely Lactobacillus plantarum, Weissella cibaria, Lactobacillus fabifermentans, Weissellahellenic and Psychromonas arctica as well as three fungal species i.e., Candiada tropicalis, Campylobacter mucosalis andCandida albicans. The predominant bacteria were Lactobacillus plantarum, Weissella cibaria and the predominant funguswas Candiada tropicalis.

Key words: Jinzhou pickles, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), diversity

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