食品科学 ›› 2017, Vol. 38 ›› Issue (2): 109-114.doi: 10.7506/spkx1002-6630-201702018

• 生物工程 • 上一篇    下一篇

极大螺旋藻高光合速率藻种的紫外诱变筛选

杨生辉,祖廷勋,罗光宏,王丹霞,陈天仁,高自成   

  1. 1.河西学院凯源生物中心,甘肃省微藻工程技术研究中心,甘肃省河西走廊特色资源利用重点实验室,甘肃 张掖 734000; 2.张掖市生产力促进中心,甘肃 张掖 734000
  • 出版日期:2017-01-25 发布日期:2017-01-16
  • 基金资助:
    2014年甘肃省农业领域知识产权优势企业培育项目(14OPIPG002);甘肃省工程技术研究中心专项(1009FTGG017);河西学院科研创新与应用校长基金项目(XZ2011-08);张掖市科技重大专项(144JTCG254-08)

UV-Induced Mutagenesis and Screening of Spirulina maxima for High Photosynthetic Rate

YANG Shenghui, ZU Tingxun, LUO Guanghong, WANG Danxia, CHEN Tianren, GAO Zicheng   

  1. 1. Kai-yuan Biotechnology Center, Gansu Engineering Research Center for Microalgae, Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye 734000, China; 2. Zhangye Productivity Promotion Center, Zhangye 734000, China
  • Online:2017-01-25 Published:2017-01-16

摘要: 目的:筛选高光合速率极大螺旋藻藻种。方法:结合自然分离筛选、超声处理、紫外诱变和高CO2条件胁迫等方法进行诱变筛选。结果:得到3 株极大螺旋藻突变株KYZ1、KYZ2、KYZ3,通过藻丝形态、生长量、CO2利用率、蛋白质含量、叶绿素含量等因素对比,并采用综合评分法,筛选出1 株优质藻株KYZ2。结论:与出发藻株相比具有藻丝长度均明显变长、藻丝个体大、生长速度快、光合速率高、蛋白质和叶绿素含量高等优点,是一株具有工业养殖潜力的藻株。

关键词: 极大螺旋藻, 紫外诱变, 突变藻株

Abstract: Purpose: To screen a mutant strain of Spirulina maxima with high photosynthetic rate. Methods: The mutagenesis and screening were conducted by a combination of spontaneous isolation, ultrasonic treatment, UV irradiation and extremely high CO2 stress. Results: Three mutants, namely KYZ1, KYZ2 and KYZ3, were obtained. The filament morphology, biomass, CO2 availability, and protein and chlorophyll contents of these three mutants were compared. Finally, KYZ2 was screened as the best strain. Conclusions: Strain KYZ2 revealed longer and larger filaments, faster growth, higher photosynthetic rate, and higher contents of protein and chlorophyll compared with the starting strain. Thus, the mutant strain could have potential for industrial culture.

Key words: Spirulina maxima, UV irradiation, mutant strain

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