食品科学 ›› 2017, Vol. 38 ›› Issue (2): 191-197.doi: 10.7506/spkx1002-6630-201702031

• 成分分析 • 上一篇    下一篇

气相色谱-质谱联用和电子鼻用于鉴别鸡精调味品香气成分

田怀香,张雅敬,吴 譞,秦 蓝,陈 臣,肖立中,于海燕   

  1. 1.上海应用技术大学香料香精技术工程学院,上海 201418;2.雀巢研发中心上海有限公司,上海 201812; 3.上海应用技术大学计算机科学与信息工程学院,上海 201418
  • 出版日期:2017-01-25 发布日期:2017-01-16
  • 基金资助:
    上海应用技术学院协同创新基金-跨学科、多领域合作研究专项(XTCX2015-13);校企合作项目(J2015-218)

Identification of Flavor Components of Chicken Bouillon Flavor Components by Gas Chromatography-Mass Spectrometry and Electronic Nose

TIAN Huaixiang, ZHANG Yajing, WU Xuan, QIN Lan, CHEN Chen, XIAO Lizhong, YU Haiyan   

  1. 1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China; 2. Nestlé R&D Centre Shanghai Co. Ltd., Shanghai 201812, China; 3. School of Computer Science and Information Engineering, Shanghai Institute of Technology, Shanghai 201418, China
  • Online:2017-01-25 Published:2017-01-16

摘要: 以新鲜鸡精、久置鸡精和哈败鸡精为研究对象,采用顶空固相微萃取-气相色谱-质谱联用技术分析其挥发性风味成分,并利用感官评价和电子鼻技术对鸡精样品的风味属性进行评定,并采用主成分分析法和聚类分析法研究电子鼻对鸡精的区分能力,采用偏最小二乘法建立挥发性风味成分与嗅闻感官属性之间的相关性。结果表明,鸡精调味品共检测出53 种挥发性风味成分,主要包括烯类3 种、醇类6 种、酮类4 种、醛类12 种、酯类6 种、芳香族化合物3 种、含硫化合物13 种、杂环化合物4 种和其他化合物2 种;人工感官评价和电子鼻结果一致,认为新鲜鸡精与久置鸡精的风味更为接近,与挥发性风味物质含量结果相吻合;偏最小二乘法结果表明,有30 种挥发性风味成分与嗅闻感官属性具有较好的相关性。研究结果表明气相色谱-质谱、电子鼻结合化学计量学方法可以综合评价鸡精调味品的嗅觉风味。

关键词: 鸡精, 挥发性风味物质, 气相色谱-质谱联用, 电子鼻, 化学计量学方法

Abstract: The volatile components of fresh chicken bouillon, out-of-date chicken bouillon and rancid chicken bouillon were extracted by headspace solid-phase micro-extraction (SPME) and identified by gas chromatography-mass spectrometry (GC-MS). Sensory evaluation and an electronic nose were used to evaluate the flavor attributes of chicken bouillons. The ability of the electronic nose to differentiate chicken bouillons was evaluated by principal component analysis (PCA) and cluster analysis (CA). The correlation between flavor components and electronic nose was established by partial least squares (PLS). The results showed that a total of 53 flavor compounds were identified in chicken bouillons, including 3 alkenes, 6 alcohols, 4 ketones, 12 aldehydes, 6 esters, 3 aromatic compounds, 13 sulfides, 4 heterocyclic compounds and 2 other compounds. The results of sensory evaluation agreed with the electronic nose results. The flavor of fresh chicken bouillon was regarded to be more similar to that of out-of-date chicken bouillon, and they were also similar in terms of volatile composition. PLS showed that 30 flavor components exhibited good correlation with the electronic nose sensors. These findings indicated that combination of GC-MS and electronic nose with chemometrics could evaluate the olfactory flavor of chicken bouillons.

Key words: chicken bouillon, flavor components, gas chromatography-mass spectrometry (GC-MS), electronic nose, chemometrics method

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