常见食源性致病菌CRISPR系统结构与功能研究进展

doi:10.7506/spkx1002-6630-201701046

食品科学

常见食源性致病菌CRISPR系统结构与功能研究进展

刘伟奇1，王旭1，刘海泉1，潘迎捷1,2,3，赵勇1,2,3,*

1.上海海洋大学食品学院，上海 201306；2.上海水产品加工及贮藏工程技术研究中心，上海 201306； 3.农业部水产品贮藏保鲜质量安全风险评估实验室（上海），上海 201306

出版日期:2017-01-15 发布日期:2017-01-16

Advances in Structures and Functions of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in Common Foodborne Pathogenic Bacteria

LIU Weiqi1, WANG Xu1, LIU Haiquan1, PAN Yingjie1,2,3, ZHAO Yong1,2,3,*

1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Shanghai Aquatic and Storage Engineering Technology Research Center, Shanghai 201306, China; 3. Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China

Online:2017-01-15 Published:2017-01-16

摘要/Abstract

摘要： 近年发现细菌和古细菌中广泛存在规律成簇间隔的短回文重复序列（clustered regularly interspaced shortpalindromic repeats，CRISPR）系统，该系统能够有效地防御噬菌体等外源基因元件的入侵，限制基因的水平转移。而在食源性致病菌中CRISPR系统除了免疫防御功能以外，还可能具有调节细菌毒力等的其他功能。并且食源性致病菌的CRISPR系统呈现出的多样性在不同菌株之间存在差异，这种差异为食源性致病菌的遗传进化、分子分型提供了更为可靠的依据。因此，近年来针对食源性致病菌中CRISPR系统结构与功能的研究逐渐成为热点。为了进一步探究CRISPR系统在食源性致病菌中的作用机制与应用前景，本文综述了几种常见的食源性致病菌CRISPR系统的基本结构及相关功能的研究进展。

关键词: 食源性致病菌, 规律成簇间隔的短回文重复序列, 结构, 功能

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) have been recently identified in most archaea
and many bacteria. This system can effectively defend against invading foreign nucleic acids derived from bacteriophages
or exogenous plasmids, limiting horizontal gene transfer. Also, the function of participating in the regulation of bacterial
virulence has been found in the CRISPR system of foodborne pathogens. There are differences in the diversity of CRISPR
in foodborne pathogens among different strains, which provides a more reliable foundation for the genetic evolution and
molecular typing of foodborne pathogens. Therefore, the structures and functions of CRISPR in foodborne pathogens
have attracted great attentions. To investigate their mechanisms of action and application prospects, recent advances in the
development of the basic structures and functions of CRISPR in common foodborne pathogens are reviewed in this article.

Key words: foodborne pathogenic bacterium, clustered regularly interspaced short palindromic repeats (CRISPR), structure, function

中图分类号:

TS201.3

刘伟奇，王旭，刘海泉，潘迎捷，赵勇. 常见食源性致病菌CRISPR系统结构与功能研究进展[J]. 食品科学, doi: 10.7506/spkx1002-6630-201701046.

LIU Weiqi, WANG Xu, LIU Haiquan, PAN Yingjie, ZHAO Yong. Advances in Structures and Functions of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in Common Foodborne Pathogenic Bacteria[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201701046.

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常见食源性致病菌CRISPR系统结构与功能研究进展

Advances in Structures and Functions of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in Common Foodborne Pathogenic Bacteria

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